Minifors 2 - Benchtop Bioreactor
Before Cultivation
Page
94
of
214
07 February 2019
Below is a detailed description of how reagent bottles are equipped
properly and connected to the pumps and culture vessel.
Connecting the reagent bottles to the pumps and culture ves-
sel
To connect the reagent bottles with the pumps and the culture ves-
sel, proceed as follows:
Procedure
1.
Cut two long silicone hoses (Ø = 2 x 6 mm) per pump/reagent
bottle.
INFORMATION
The length of the silicone hoses must be selected to ensure that
the hose connections between the reagent bottles, pumps and
culture vessel do not have any tensions or kinks
.
2.
Thoroughly rinse the silicone hoses with distilled water.
3.
Connect the silicone hoses and pump hoses of the pump
heads with hose connectors.
For the
Fill
function:
Right-hand side = suction side = hose line to reagent
bottle.
Left-hand side = pumping side = hose line to culture
vessel.
See arrow for direction of rotation
.
4.
Secure with cable ties.
Connection between pumps and culture vessel
Procedure
1.
Fit silicone hoses for base, acid and feed to addition port
adapter and/or feed needle(s) and secure them with cable
ties.
