Minifors 2 - Benchtop Bioreactor
Setup and Function
Page
36
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214
07 February 2019
3.6.1 Gas Entry
A silicone hose leads the gas or gas mixture from the gassing con-
nection on the basic unit to the culture vessel, via a sterile filter.
The gas is fed directly into the medium via the sparger (sparger
gassing). For more details on the sparger, see the “Accessories”
section, sub-section “Sparger”.
3.6.2 Exit Gas
Even without active gassing, any cultivation can increase the pres-
sure inside the vessel through heating or gas production. As such,
an exit gas line is essential for all cultivation processes.
Siphoning off exit gas via the exit gas cooler
The exit gas cooler dries the exit gas through condensation, thus
preventing the exit gas filter from becoming clogged with moisture.
At the same time, it also prevents liquid loss in the culture medium.
INFORMATION
If heavy foaming is expected, a bottle of antifoam agent can be
installed upstream of the exit gas filter as a foam trap.
The exit gas cooler is included in the standard package; for more
details, see the “Accessories” section, sub-section “Exit Gas
Cooler”.
3.7 pH Control
pH in the medium is measured by the pH sensor and regulated by
addition of reagents (acid, base). Addition of acid and base takes
place via the two peristaltic pumps
Acid
and
Base
.
Reagent bottles are filled with acid and base which are connected
to an addition port adapter in the vessel top plate and the two
pumps by silicone hoses.
3.7.1 Measurement System
The pH measurement system is designed for use with HAMILTON
digital sensors:
Conventional pH sensor (potential measurement against refer-
ence) with built-in electronics
