Minifors 2 - Benchtop Bioreactor
Before Cultivation
07 February 2019
Page
107
of
214
8.2.11 Calibrating the pO
2
Sensor
Generally speaking, the following applies: The pO
2
sensor should
be calibrated after autoclaving has been performed because the
sterilisation process may change the steepness of the pO
2
sensor.
As a rule, a 1-point calibration to 100 % is usually sufficient for ex-
act measurement, and should be carried out before each cultiva-
tion.
Depending on the specifications defined by the user, the pO
2
sen-
sor is calibrated either before the vessel is filled with medium or
afterwards, in the prepared medium.
For more details on the calibration refer to main chapter “Opera-
tion”, chapter “Calibrating the pO
2
Sensor”.
8.2.12 Checking the Hoses and Hose Connections
Check and ensure the following items before each cultivation:
Hoses show no kinks and are not able to kink.
Hoses are undamaged and show no weaknesses.
Gas hoses and connections do not show any leaks.
Hose lines are as short as possible.
Hoses are secured with cable ties and/or hose clamps.
Only the pressure hoses supplied by the equipment manufac-
turer are connected as supply lines (water, gas) between the
in-house connections and the equipment.
