Minifors 2 - Benchtop Bioreactor
Before Cultivation
Page
98
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214
07 February 2019
8.1.26 Fitting the Cone Plug for Drive Hub
In order to prevent the penetration of condensation water into the
dive hub during autoclaving, the cone plug provided in the starter
set must be fitted.
ATTENTION
Risk of loss of property du penetration of condensation water
into the drive hub!
Always autoclave the culture vessel with the cone plug fitted to
the drive hub!
Proceed as follows:
Procedure
1.
Plug the cone plug into the opening of the drive hub.
8.1.27 Checklist Before Autoclaving
Check and ensure the following items before autoclaving:
Culture vessel
All necessary O-rings are fitted.
All unused ports are closed with blanking plugs
Connection for inoculation is equipped with septum, septum collar and blanking plug
Drive hub
is equipped with
cone plug
.
There is liquid in the culture vessel (autoclavable medium or approx. 10 mL water per litre working volume).
Reagent bottles, hoses and pumps
Reagent bottles are exclusively filled with autoclavable reagents, correctly labelled and connected with the
culture vessel and the pump heads via hoses.
Reagent bottles are equipped with filters for pressure equalisation
