Minifors 2 - Benchtop Bioreactor
Operation
Page
176
of
214
07 February 2019
12.
Wait until the medium is saturated with oxygen, i.e. wait until
the measured value (display via CALIBRATE POINT 2) is sta-
ble.
13.
Press
CALIBRATE POINT 2
(or 1) to begin calibration.
Gassing and the stirrer are stopped.
CONFIRM NOW
creates a visual representation of the calibra-
tion procedure and slowly turns orange.
The procedure takes 3 minutes to guarantee a stable value.
When the countdown has finished, CONFIRM NOW is com-
pletely coloured in.
INFORMATION
To skip the waiting time, the same conditions apply as described
in the first calibration point.
14.
If the calibration was successful, confirm it with
OK
and leave
the menu.
10.8.6 Calibrating the Turbidity Sensor - General Information
Optek turbidity sensors (optional) are pre-calibrated in the factory.
Inserts are available for reference measurement.
Due to the different light absorption of different media, zero point
calibration of the turbidity sensor should be performed before each
cultivation process. This can be done either
before or after
sterilis-
ing, depending on the application in question.
Conditions for zero point calibration of the sensor
The sapphire windows of the optical density sensor must be clean
and free of air or gas bubbles.
The light absorption of the medium before activation of the gassing
and before inoculation can be used as a reference value for the
zero point.
10.8.7 Calibrating the Turbidity Sensor - Procedure
Proceed as follows to calibrate the zero point of the (optional) tur-
bidity sensor:
Procedure
1.
Connect the sensor cable.
2.
Call up the calibration menu of the
Turbidity
parameter.
