Minifors 2 - Benchtop Bioreactor
Cultivation
07 February 2019
Page
113
of
214
All clamps have been removed (except for sampling system).
Utensils for the inoculation and vessels with inoculum are
ready.
Methods
There are a number of ways to add medium or inoculum before
and during cultivation:
In a small volume, with the syringe via the septum
Via the addition port adapter from the reagent bottle (a sterile
hose connection is required for this method).
Via the dip tube from the reagent bottle (a sterile hose con-
nection is required for this method).
These methods are described below.
The implements for inoculation with a syringe are standard acces-
sories for the equipment. This inoculation method is particularly
suitable for all vessel sizes of the equipment.
9.3.1 Inoculation with a Syringe
Proceed as follows for the inoculation:
Procedure
1.
Fill the syringe with the required amount of inoculum.
2.
Unscrew the blanking plug from the septum collar.
As a possible additional protection against contamination:
Before piercing, drop a few drops of ethanol (70 %) on the
septum.
3.
Pierce the septum and inject the inoculum.
4.
Remove the needle from the septum and close the septum
collar with a blanking plug.
9.3.2 Inoculation Using Dip Tube / Addition Port Adapter
Proceed as follows during inoculation:
Procedure
1.
Fill the inoculum under sterile conditions into the prepared
container.
2.
Create a sterile hose connection with the dip tube/addition
port adapter.
3.
Transfer the desired volume of inoculum into the culture ves-
sel. Pump it, if necessary.
4.
Clamp off the hose with the hose clamp, weld it if necessary.
