Minifors 2 - Benchtop Bioreactor
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07 February 2019
4.2 Exit Gas Analysis
In order to allow the user to draw conclusions regarding the status
of the culture while the bioprocess is still underway, the CO
2
and
O
2
measurements are often taken and analysed in the exit gas flow
of the bioreactor.
4.2.1 Gas Sensors
Combined CO
2
and O
2
gas sensors of the type BlueInOneFerm or
Blue Vary from the manufacturer BlueSens are available for exit
gas analysis.
3 m of pressure hose, D = 8 x 14.5 and a clamp are included for
connecting the gas sensor to the culture vessel (exit gas filter).
Measurement ranges gas sensors
Vol. % O2
Vol. % CO2
1.0 – 50
0 – 10
or
0 – 25
INFORMATION
Both sensor types are only suitable for use in aerobic bopro-
cesses!
For details on the safety, technical data, usage and maintenance
requirements for the gas sensors, see the separate documentation
provided by the sensor manufacturer. Read this documentation
before using the gas sensor and follow the instructions contained
therein.
4.2.2 Connecting the Gas Sensor
In order to view measurements on the operating panel, you must
connect the gas sensor to the sensor cable and channel the exit
gas from the bioreactor through the gas sensor using a hose. The
cable is usually connected once during commissioning and can
remain untouched thereafter. The connection to the exit gas line
must be re-established before each cultivation process.
The ideal connection conditions are detailed in the separate docu-
mentation provided by the manufacturer.
