Minifors 2 - Benchtop Bioreactor
Before Cultivation
Page
104
of
214
07 February 2019
To couple the motor, proceed as follows:
Procedure
1.
Place the motor onto the drive hub with the groove aligned
with the pin on the drive hub.
The motor is held in its position.
8.2.6 Filling the Culture Vessel
Depending on the application, the vessel can be filled after auto-
claving. To prevent foam formation during filling, add the medium
via a dip tube.
To do so, proceed as follows:
Procedure
1.
Sterilise the medium separately.
2.
If necessary, pump off any water that remains in the culture
vessel.
3.
Establish a sterile hose connection between the culture vessel
and the medium container.
4.
Pump the desired quantity of medium into the culture vessel.
5.
Clamp off the medium hose; if necessary, apply a welded
seal.
6.
Disconnect the medium container from the culture vessel; if
necessary, retain it as a harvest or waste container.
INFORMATION
If the stirrer is turning on the surface of the medium, foam will be
formed. For this reason, only switch on the stirrer if it is fully cov-
ered by medium.
