Minifors 2 - Benchtop Bioreactor
Before Cultivation
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07 February 2019
3.
Equip the hose for a sterile hose connection. (Depending on
the application: rapid coupling, sterile connector or weldable
hose with sterile filter).
4.
Secure the hose transition points with cable ties.
8.1.14 Mounting the Exit Gas Cooler
Mount the exit gas cooler as follows:
Procedure
1.
Attach the O-ring to the thread of the exit gas cooler.
2.
Screw the exit gas cooler onto the thread in the
12 mm/Pg13.5 port by hand.
3.
Align the exit gas cooler to ensure that handling of other com-
ponent parts is impaired as little as possible.
4.
Check to ensure that the exit gas filter is fitted securely.
5.
Cap the exit gas filter loosely with a little aluminium foil
INFORMATION
A humidifier bottle with antifoam reagent can be installed be-
tween exit gas cooler and the exit gas filter if significant foam
formation is expected.
Take the following into account for autoclaving:
Only use a new, clean and dry exit gas filter and fix it in such a
way that it cannot slip.
ALWAYS keep the exit gas line - hose at the exit gas cooler
with secured exit gas filter - open.
CAUTION
If pressure equalisation does not take place via a top plate
opening or the mounted exit gas cooler, overpressure or vac-
uum in the culture vessel may occur during autoclaving.
