Minifors 2 - Benchtop Bioreactor
Installation and Initial Operation
Page
70
of
214
07 February 2019
ATTENTION
If the vessel top plate presses against long components such as
the stirrer shaft etc., they could bend because of the weight of
the top plate.
Always position the vessel top plate so that it does not lie on top
of components.
2.
Fill the culture vessel with water – preferably demineralised –
to the working level.
3.
Ensure that the stirrer and sparger are mounted; if necessary,
mount them.
4.
Fit the top plate and secure it.
5.
Screw the exit gas cooler into the port on the vessel top plate
port.
The exit gas cooler is equipped with a new exit gas filter
in the factory.
6.
Connect the exit gas cooler to pre-fitted hoses on the basic
unit; to this end, follow the symbols on the basic unit:
water inlet on bottom of exit gas cooler / water outlet at
top of exit gas cooler.
7.
Close all remaining open ports with blanking plugs.
8.
Hang the culture vessel on the basic unit.
9.
Connect the sparger to the gassing system (compressed air)
on the equipment; to this end, attach the gassing hose to the
nozzle on the inlet air filter.
The sparger is equipped with hose and the inlet air filter
in the factory.
The gassing hose is attached to the basic unit in the fac-
tory.
10.
Insert the temperature sensor as far as it will go into the
pocket in the top plate.
11.
Couple the motor.
12.
Switch on the equipment at the main switch and wait until it
has started up.
7.5.2 Cooling System
To activate the cooling system, proceed as follows:
Procedure
1.
On the operating panel, set a low setpoint for the
Temperature
parameter, e.g. 10 °C, in order to activate the water supply to
the temperature control system.
