Minifors 2 - Benchtop Bioreactor
Before Cultivation
Page
92
of
214
07 February 2019
4.
Turn the sterile filter carefully by hand in a clockwise direction.
This ensures that the non-return valve/sterile filter screw con-
nection is tight.
5.
Cover the valve group loosely with aluminium foil.
6.
Clamp off the hose on the dip tube.
8.1.21 Mounting the Sparger Hose and the Inlet Air Filter
The sparger must be equipped with the hose and inlet air filter be-
fore autoclaving.
To do so, proceed as follows:
Procedure
1.
Cut a short piece of hose:
1.5 L culture vessels: silicone hose Ø = 2 x 6 mm
3.0 L and 6.0 L culture vessels: pressure hose, transpar-
ent, Ø = 5 x 10 mm.
2.
Fit the inlet air filter to the hose piece:
1.5 L culture vessel: filter with red marking, Ø = 37 mm, fit it in
the direction of the air flow to the hose end. The nozzle with
the red
INLET
marking remains exposed.
