Applied Biosystems Data Explorer 4 Series, User Manual

Page: 257 / 447

Adjusting the Baseline
Data Explorer
™
Software User’s Guide 5-53
5
NOTE: Flexibility and Degree parameters may require
some testing to determine the optimum settings for your
data.
4. Click OK.
The baseline is adjusted, and the trace is displayed with a
AdvBC trace label.
Degree Determines how closely the calculated baseline fits the
data. Valid entries are 0.0 to 1.0.
A value closer to 1 fits the baseline more closely to the
data, and corrects the midpoint of the noise signal to
approximately 0 intensity.
A value closer to 0 fits the baseline less closely to the
data, and corrects the midpoint of the noise signal to a
value greater than 0 intensity (see below).
For protein and tryptic digest peptide spectra, use 0 to
0.5. If the baseline after correction is too high, increase
the Degree value (see below).
A lower Degree setting allows the baseline correction to
occur more quickly, and often provides better correction
than a higher setting.
Parameter Description/Specifies
Degree=1
Midpoint of noise signal
corrected to 0
Degree=0
Midpoint of noise signal
corrected above 0