Applied Biosystems Data Explorer 4 Series, User Manual

Page: 118 / 447

Chapter 3 Peak Detection and Labeling
3-20 Applied Biosystems
3
Detection Ranges
(continued)
To delete a range, select the range, then click .
To combine all ranges in the list into one range, click .
The peak detection settings displayed in the dialog box
correspond to the selected range. To view peak detection
settings for another range, select the range of interest.
%Base Peak
Intensity
NOTE: This
parameter was
previously named
Peak Threshold%
and Base Peak
Relative%.
Specifies a percentage of the base peak intensity as the
threshold value.
To be detected, peaks must be above this threshold and
above the %Max Peak Area value.
Hint: For most Voyager applications, leave this parameter
set at 0 and adjust the %Max Peak Area.
%Max Peak Area Specifies a percentage of the peak with the largest area as
the threshold value.
To be detected, peaks must be above this threshold and
above the %Base Peak Intensity value.
%Max Peak Area is calculated above the local baseline and
can compensate for problems related to a rising global
baseline and signal spikes.
Hint: If you are analyzing digest data, set %Max Peak Area
to 0 before deisotoping to ensure that all peaks of interest
are detected. For more information, see Section 7.2.3,
Detecting Peaks from Complex Digests.
Filter Width Specifies the number of data points used in smoothing for
peak detection before integration. The software uses a Filter
Width Increment of 1 when applying smoothing.
NOTE: If you set Filter Width too high, narrow peaks may not
be detected.
Continued
Table 3-1 Chromatogram Settings (Continued)
Parameter Description