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Chapter 3 Peak Detection and Labeling
3-36
Applied Biosystems
3
Effect of Minimum
Intensity
The Minimum Intensity setting on the Advanced Settings tab
can also determine how charge states are determined for a
peak, because it determines if the software can find a match
for a peak. Figure 3-12 illustrates two peaks correctly labeled
with charge state 1.
Figure 3-12 Minimum Intensity Set Correctly
If you increase the Minimum Intensity setting to 85 in the
example shown in Figure 3-12, the software cannot determine
a charge state for the first peak (Figure 3-13). This is because:
•
The increased threshold suppresses the detection of the
236.6 m/z peak.
•
When the software evaluates the peak at 235.6 m/z, it
does not find a peak at the appropriate peak spacing for
charge state 1, and therefore determines that the peak
has no calculated charge.
Figure 3-13 Minimum Intensity Set Incorrectly
Charge state
determination
troubleshooting
See Table 9-12, “Charge State and Isotope Determination
Troubleshooting—Mariner Only,” on page 9-18.
Summary of Contents for Data Explorer 4 Series
Page 1: ...Data Explorer Software Version 4 Series Software User Guide ...
Page 10: ...Table of Contents x Applied Biosystems ...
Page 56: ...Chapter 1 Data Explorer Basics 1 42 Applied Biosystems 1 ...
Page 98: ...Chapter 2 Using Chromatogram and Spectrum Windows 2 42 Applied Biosystems 2 ...
Page 172: ...Chapter 3 Peak Detection and Labeling 3 74 Applied Biosystems 3 ...
Page 204: ...Chapter 4 Examining Chromatogram Data 4 32 Applied Biosystems 4 ...
Page 270: ...Chapter 5 Examining Spectrum Data 5 66 Applied Biosystems 5 ...
Page 316: ...Chapter 6 Using Tools and Applications 6 46 Applied Biosystems 6 ...
Page 384: ...Chapter 9 Troubleshooting 9 22 Applied Biosystems 9 ...
Page 388: ...Appendix A Warranty A 4 Applied Biosystems A ...
Page 416: ...Appendix C Data Explorer Toolbox Visual Basic Macros C 20 Applied Biosystems C ...