Applied Biosystems Data Explorer 4 Series, User Manual

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Chapter 5 Examining Spectrum Data
5-20 Applied Biosystems
5
CAUTION
The software allows you to add multiple items with the
same m/z value to the reference file if any other
attribute of the reference compound is different (for
example, charge state or name). Each mass in the list
is considered during calibration. If the mass list
contains duplicate entries, the calibration may return an
invalid number of matches.
6. To modify an entry, click the reference mass in the list to
select it, modify the entry as needed, then click Update.
The modified entry is saved in the .REF file.
7. Click Save.
8. In the Save As dialog box, select a location and type a
name for the file, then click OK.
The name of the reference file used for mass calibration
is stored in the .DAT file and is displayed in the
Calibration Reference File field when you open the
Manual Calibration dialog box again.
NOTE: Only the name and path of the reference file are
stored in the .DAT file. Contents of the file are not
stored.
Modifying a
calibration
reference file
Hint: You can also modify a calibration reference file when
you manually select reference masses during calibration.
See step 8 on page 5-11.
To add or delete information in a calibration reference file:
1. From the Process menu, select Mass Calibration, then
select Edit/Create Reference File.
The Edit/Create Reference Peak Information dialog box
(Figure 5-6 on page 5-19) is displayed.
2. Click Browse, then select a calibration reference file.