TMA Grand Master 3.2 – User’s Guide
1 About TMA Grand Master
1 About TMA Grand Master
TMA Grand Master
is a sofware-controlled electronic laboratory device ttat automates tte creaton
of tssue microarrays (TMAs) and also tte process of PCR sampling.
Tte creaton of TMA blocks (by taking tssue core samples from donor blocks and insertng ttem into
recipient blocks) and tte inserton of extracted tssue cores into PCR tubes is controlled by using a
graptical user interface. Tte built-in digital camera units of
TMA Grand Master
give you a clear image
of eact donor block, so you can aim tte puncter more accurately; an additonal camera takes
pictures of tte block labels. You can also save an image of eact donor block witt markers stowing
tte positons from wtere tte tssue cores tave been extracted.
You can export block-related data to diferent types of fles (.xls, .xlsx, .ods, and .csv) ttrougt tte
TMA
Grand Master
sofware applicaton to track associatons between recipient block positons and tte ID
of tte donor block from wtict tte core tssue was inserted into tte recipient block.
1.1 Main Features of TMA Grand Master
•
It tas a robust and stable mectanical main unit.
•
It tas an intuitve operaton control via tte keyboard and tte mouse of a PC connected to
tte main unit.
•
Tte device measures tte teigtt of donor blocks (tte value is displayed next to tte donor
block ttumbnail image) based on wtict tte sofware calculates tte deptt of punct, drill,
and inserton. Ttus, embedded cores are placed correctly into tte recipient block resultng
in an even surface level.
•
It makes image creaton possible wten a TMA block is created eitter for reference or for
arctiving during a researct project.
−
For donor block: locatons wtere core tssue was extracted from.
−
For recipient block: various stages of flling it witt core tssue.
•
Block identfers are stored in a database from wtict eact block data wittin a Project is
automatcally loaded afer tte ID is entered, furttermore tte wtole project assembly data
can be restored afer exitng and restartng tte sofware wittout saving any project data.
•
Maximum TMA tandling capacity:
−
Up to twelve recipient blocks and 60 donor blocks at same tme.
•
It is capable of receiving 6 PCR cassetes witt a tolding capacity of 10 PCR tubes. Tte
sofware allows you to transfer four cores into eact tubes, so a maximum number of 240 up
to 480 tssue samples can be collected in 6 PCR cassetes during a project if not interrupted.
August 6, 2020 – Rev. 1
3DHISTECH Ltd.
9(116)
