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3DHISTECH TMA Grand Master 3.2, User Manual

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3DHISTECH TMA Grand Master 3.2 User Manual Download Page 12

TMA Grand Master 3.2 – User’s Guide

2 TMA Grand Master Hardware

Back  iew

Figure 2 – TMA Grand Master back view

1. Terminal point for tte power supply cord

2. Main power supply switct

3. Fuse compartment

4. USB ports to connect witt tte computer.

2.2  Cablinn

For required cable connectons, tte supplied cables must be plugged in as stown in tte fgure above.

To connect tte main unit witt tte computer any of tte ttree USB ports can be used.

 Warninn!

All cable connectons must be ctecked for frm seatng. Defectve cables must not be 
connected to any part and must be replaced witt cables in proper operatng conditon. 

Contact 3DHISTECH support at [email protected] in suct cases.

 Important!

Tte use of an uninterruptble power supply (UPS) is recommended but optonal.

August 6, 2020 – Rev. 1

3DHISTECH Ltd.

12(116)

Summary of Contents for TMA Grand Master 3.2

Page 1: ...GM32 UG EN Rev1 TMA Grand Master 3 2 User s Guide August 6 2020 Rev 1 ...

Page 2: ... Switcting tte Main Unit On or Of 20 4 2 Startng tte TMA Control Applicaton 20 4 3 Main User Interface 23 4 3 1 Main Toolbar 24 4 4 Insertng and Removing Blocks 27 4 4 1 Block preparaton 27 4 4 2 Cleaning block and PCR cassete preparaton 28 4 4 3 Insertng a block into tte main unit 29 4 4 4 Removing a block from tte main unit 31 4 4 5 Removing a PCR cassete from tte main unit 31 4 5 Ctanging tte D...

Page 3: ...reventve Maintenance of tte Main Unit 91 7 2 Cleaning Tasks 91 7 2 1 Opening tte Service Compartment Door 92 7 3 Replacing fuses of tte main power switct 93 7 4 Contaminated Tray or Touct Sensor Surface 94 8 Troubleshootnn TMA Grand Master 96 8 1 Hardware related issues 96 8 2 Sofware related issues 97 9 Cassete and Block Dimensions 100 10 Technical Descripton of the Hardware 101 10 1 Ptysical Dim...

Page 4: ...U04 00019 EP 1644767 US 7327515 Tte above mentoned granted patents granted utlity model registered trademarks pending internatonal and natonal patent applicatons and tte otter corresponding IP are tte sole propertes of tte 3DHISTECH Ltd 3DHISTECH Ltd is not liable for damane of whatever nature includinn but not limited to neneral or specific damane indirect damane consequental damane or incidental...

Page 5: ... manufacturer could void the user s authority to operated the equipment under FCC rules CAUTION For researct and educaton uses only not for use in diagnostc procedures Ttis product tas not been approved or cleared as a medical device by tte U S Food and Drug Administraton nor any otter regulatory auttority Tte data and images obtained or viewed using ttis product are not intended for clinical or d...

Page 6: ...1 General requirements IEC 61010 2 101 2002 ed 1 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Partcular requirements for in vitro diagnosti IVD medical equipment DIN EN 55011 2009 Class B Limits and mettods of measurement of radio disturbance ctaracteristcs of industrial scientfc and medical ISM radio frequency equipment Ttis product tas been t...

Page 7: ...O key combinaton Example Cross references to otter documents or sectons wittin ttis document Warninn Indicates ttat you need an additonal module to use tte feature ttere are prerequisites for tte task or ttat special care stould be taken wten using certain feature of tte device Important An important piece of informaton or a recommendaton Tte applicaton will work if you ctoose not to follow tte re...

Page 8: ...ock Tte tssue cores are removed from tte donor and inserted into a recipient parafn block Clone Block A duplicate triplicate quadruplicate etc of a recipient block witt identcal layout and core order TMA Tissue microarrays Tte tssue microarray consists of an array of cylindrical cores of parafn embedded tssue ttat are removed from preexistng donor parafn blocks PCR Polymerase Ctain Reacton a bioct...

Page 9: ...stable mectanical main unit It tas an intuitve operaton control via tte keyboard and tte mouse of a PC connected to tte main unit Tte device measures tte teigtt of donor blocks tte value is displayed next to tte donor block ttumbnail image based on wtict tte sofware calculates tte deptt of punct drill and inserton Ttus embedded cores are placed correctly into tte recipient block resultng in an eve...

Page 10: ... witt tte overlay positoning You can enable ttis opton witt tte Use ttree reference points buton on tte Advanced Manual Overlay Setngs window Using ttree reference points will distort tte overlay slide TMARenister CaseCenter lonin and Settinnsn TMARegister got its own Setngs window and Login buton If you open tte TMARegister from TMAControl and you are already logged in to tte server you do not ta...

Page 11: ... and tte control computer Accessories box Blocks Base molds for parafn blocks 2 1 TMA Grand Master Main Unit Front iew Figure 1 TMA Grand Master front view 1 Protectve tousing 2 Door opener buton 3 Tool ctange compartment door 4 Protectve window 5 Trast container for parafn nakes and discarded core material 6 Grips for service compartment door 7 Front power supply switct August 6 2020 Rev 1 3DHIST...

Page 12: ...ables must be plugged in as stown in tte fgure above To connect tte main unit witt tte computer any of tte ttree USB ports can be used Warninn All cable connectons must be ctecked for frm seatng Defectve cables must not be connected to any part and must be replaced witt cables in proper operatng conditon Contact 3DHISTECH support at support 3dtistect com in suct cases Important Tte use of an unint...

Page 13: ...stc box drill bits puncters and pistons parafn block base molds accessories and tools for tte assembly Tte siize and tte content of tte box can vary according to your purctase order For furtter informaton on tte basic accessories see secton 10 2 Accessories Important Accessories are classifed as consumable goods tterefore warranty cannot be applied to ttese items Tte life span of tools tigtly depe...

Page 14: ...l Microsof Excel or OpenOfce on tte control computer 2 4 Installinn and Confinurinn TMA Grand Master Hardware Warninn Only qualifed personnel may install TMA Grand Master and perform tte inital start up procedure 3DHISTECH Service tectnicians install and confgure TMA Grand Master and tand it over to you in operatng conditon 2 5 Transportnn the TMA Grand Master Main Unit Warninn If you need to move...

Page 15: ...ide plates of tte main unit Figure 4 Transporting handle 4 Place tte device into tte designated cardboard box onto tte botom protectve foam 5 Arrange protectve padding on tte four top corners 6 Pack tte accessories into tteir box 7 Put tte main unit and tte accessory box to tteir designated places in tte road box 8 Close tte box and tape it August 6 2020 Rev 1 3DHISTECH Ltd 15 116 ...

Page 16: ...se and for storing digital slides TMA Control Sofware Installaton Important 3DHISTECH service tectnicians perform tte installaton and confguraton of tte TMA Control sofware Tte following installaton procedure is for informaton purposes only follow tte below steps only if you need to re install tte sofware or upgrade to a newer sofware version Warninn Tte control PC on wtict tte SW applicaton of TM...

Page 17: ...1 Launct tte TMAControl_3 2_RTM_x64 exe fle ttat you received on a 3DHISTECH Installaton Disc or downloaded in a compressed fle 2 Follow tte instructons in tte installaton wiizard 3 Accept tte End User License Agreement and click Next August 6 2020 Rev 1 3DHISTECH Ltd 17 116 ...

Page 18: ...rol Software 4 Tte installer notfes you if it cannot fnd tte prerequisites Click Install to start tte installaton of missing prerequisites 5 Click Next to contnue witt tte installaton of tte TMA Control sofware applicaton August 6 2020 Rev 1 3DHISTECH Ltd 18 116 ...

Page 19: ...elect tte type of license browse for tte license fle or select HASP dongle 7 Ctoose tte installaton folder for tte TMA Control sofware applicaton Click Browse for locatng a diferent folder ttan tte default one tten click Next to contnue August 6 2020 Rev 1 3DHISTECH Ltd 19 116 ...

Page 20: ...ontrol click Finish to close tte wiizard Important Afer tte installaton RW access rigtt to database and confguraton fle must be granted for Admin and Standard users Important Tte Slide Converter sofware MUST be installed on your computer to utliize tte Spot Slide savinn functon of tte TMA Control sofware August 6 2020 Rev 1 3DHISTECH Ltd 20 116 ...

Page 21: ...plicaton on tte control computer 2 Switct of tte TMA Grand Master 3 Turn tte control computer and tte monitor of 4 2 Startnn the TMA Control Applicaton You can start tte program by double clicking onto tte program icon of tte desktop Warninn If you start tte sofware but tte tardware is turned of a warning message appears Switct on tte main unit tten click Retry in tte message box Warninn If you re...

Page 22: ... TMA Control sofware If you start tte applicaton for tte frst tme a New Project window is displayed in wtict you must enter a name for tte project If you already tave worked on projects previously and you want to create a new project click New and if you want to load a project ttat tas been saved previously click Load in tte Project panel ttat is located at tte top rigtt corner of tte applicaton w...

Page 23: ...vatng Date Ranne and or Diameter flters Wten writng tte name of tte project to be loaded suggested projects appear on tte list according to tte entered text string According to tte database driven project tandling eact tme you restart tte sofware even wittout saving tte current process of tte actual project before exitng all tte data is loaded automatcally to restore tte previous state August 6 20...

Page 24: ...rocessing mettod of blocks tte normal view includes ttumbnails of all recipient tray positons and as for donor blocks tte selected tray is displayed in its full capacity Tte otter view mode telps users to identfy blocks and tteir statuses more easily Click at tte top of recipient blocks panel and at tte rigtt end of donor blocks panel to switct to ttis view August 6 2020 Rev 1 3DHISTECH Ltd 24 116...

Page 25: ...r block was loaded into a slot on a donor block tray its image will appear on tte lef secton of tte donor blocks panel at tte botom of tte main applicaton window At tte donor panel a separator can be dragged sideways and is a used for dividing donor block panel to display TMA block ttumbnails ttat are being processed and ttat are fnisted 4 3 1 Main Toolbar Tte main toolbar of tte TMA Control sofwa...

Page 26: ...ages Layout PCR Setngs Barcode Tteme and Language See ctapter 6 Settgs for more informaton About window contains details of TMA Control sofware and includes tte following panels Info displays sofware version informaton and licensed features User Guide Manuals of TMA Grand Master TMA Master and TMA Master II can be accessed and opened directly by clicking tte links August 6 2020 Rev 1 3DHISTECH Ltd...

Page 27: ...tte links NOTEn Before opening tte videos make sure to copy video fles to C Program Files 3DHISTECH TMA Control Videos TMA Grand Master folder from tte installaton disk Ttis folder may be tidden and ttus requires auttentcaton Links Company and product web page links are displayed tere Exit applicaton August 6 2020 Rev 1 3DHISTECH Ltd 27 116 ...

Page 28: ...may end up breaking tte puncter needle Important To ensure tte good quality of recipient blocks make sure ttat tte top surfaces of bott recipient and donor blocks are parallel to tte plastc botom of tte cassetes and ttere are no dents or cavites in tte parafn layer If any defect is visible cut tte top layer of tte block to make it even otterwise embedded cores may be damaged afer creaton for examp...

Page 29: ... cassete clean tte slot area and tte cassete tten insert tte cassete again For cleaning blocks use regular 37mm siize blocks only To avoid contaminaton cleaning block are used before eact PCR sampling A cleaning block is a virgin recipient block from wtict parafn core is extracted and placed back witt tte puncter tence cleaning its surface Upon selectng tte cleaning block on tte user interface an ...

Page 30: ...cordingly Tte number of cleaning spots depending on tte puncter diameter are tte following Puncher diameter Number of cleaninn spots 0 6mm 252 1mm 144 1 5mm 72 2mm 50 4 4 3 Insertnn a Block into the Main Unit 1 Recipient block positons are displayed in a double column on tte lef side of tte screen Tray positons of donor blocks are identfed by six butons A F tray capacity is 10 blocks eact August 6...

Page 31: ...e main toolbar Tte ligtt pulsates for about 10 seconds and it is possible to open tte door wittin ttis tme frame countdown is displayed in place of tte buton 5 Pust tte door opener buton to open tte door 6 Press down tte nap in front of tte block slot 7 Slide tte block into positon witt tte slanted side towards tte back of tte mactine 8 Release tte nap wten tte block is in positon 9 Close tte door...

Page 32: ...n area 5 Pust tte door opener buton to open tte door 6 Press down tte nap in front of tte block slot 7 Remove tte block from its positon and release tte nap 8 Close tte door 4 4 5 Removinn a PCR Cassete from the Main Unit Warninn Afer removing PCR cassetes make sure ttat cores are not clung to tte port edges of tte cover plates Before carefully removing tte cover plate of a PCR cassete gently tap ...

Page 33: ...tool exctanger compartment door Figure 7 Tool compartment 4 Loosen tte drill ctuck locking lever marked witt red ttat fxes tte drill ctuck in positon and remove tte drill ctuck by lifing it upwards Figure 8 Removing the drill chuck August 6 2020 Rev 1 3DHISTECH Ltd 33 116 ...

Page 34: ...nwards as muct as you can 7 Hold tte front cog wteel frmly and tgtten tte rear cog wteel clockwise Make sure witt tte telp of tte tool ttat tte upper cog wteel is secured frmly 8 Insert tte drill ctuck into its positon and fx it witt tte locking lever Ensure ttat tte two cog wteels motor and drill ctuck are aligned exactly 9 Loosen tte puncter ctuck locking lever ttat keeps tte puncter ctuck in po...

Page 35: ...ter into tte puncter ctuck matcting tte siize of tte piston Tigtten tte rear cog wteel tolding tte front cog wteel frmly 12 Insert tte puncter ctuck into its positon and fx it witt tte puncter ctuck locking lever Ensure ttat tte two cog wteels motor and puncter ctuck are aligned exactly and move tte piston into positon making atenton to place tte piston collar to tte groove on tte console 13 Close...

Page 36: ...d blocks you can place additonal blocks and mark drilling positons on ttem so you can work simultaneously witt tte TMA Grand Master You can create up to twelve identcal TMA blocks by taking out one tssue core sample and insertng it into eact recipient block Tten you can eitter create several slides from tte TMA blocks or you can just keep one of tte TMA blocks as a backup Wten you are flling tte r...

Page 37: ...tes witt tte parafn blocks are properly inserted into tte slots Make sure ttat tte drill bit is of appropriate siize and ttat it is fxed in its positon For more informaton see secton 11 Cassete atd Block Dimetsiots 5 2 Workinn with Recipient Block Layouts 5 2 1 Creatnn a Recipient Block Layout To create a layout click tte arrow or at tte upper secton of tte Layout buton next to tte recipient block...

Page 38: ...cannot be modifed and will be applied for tte layout to be created Set tte number of Columns Rows and Gap value in tte Grid propertes secton NOTEn Afer inserton tte puncter moves sideways to break tte core As ttis movement is not possible in some cases wittout contactng witt an already inserted core please raise tte Gap value to prevent contaminaton and tte core from being damaged If setng a lower...

Page 39: ... tte layout can be divided into several sectors Mark an area wittin tte array witt tte mouse by drawing a border around tte group of inserton positons Sector area can be modifed by dragging tte marking border to tte desired directon and tte entre selecton area can be moved to a desired directon by dragging Tte selected sector can be deleted by clicking tte buton at tte top of tte border August 6 2...

Page 40: ... a tme tten tte device processes tte upcoming sector you tave created It is available for you to create a TMA block on wtict tte sectors are separated by leaving out rows and columns between tte sectors by selecton Tte dividing areas can be selected by drawing witt tte rigtt mouse buton ttus turning ttose spots inactve August 6 2020 Rev 1 3DHISTECH Ltd 40 116 ...

Page 41: ...ncton and set tte correct measurements Patern alignment can be set at tte Alinnment pane of tte window Select tte desired mettod of flling for tte specifc layout under Fillinn Directon Tte icon represents random flling as a flling mettod for blind studies NOTEn Wten using tte Sequental Scorinn functon of tte TMA Module tte toriizontal default flling mettod can be applied only otters will not work ...

Page 42: ...directon is presented at tte top lef corner of tte recipient block panel For more informaton on layout labeling see ctapter 6 Settgs Ltiayout 5 2 2 Modifyinn a Recipient Block Layout 1 Open Layout Editor window by clicking tte or tte buton next to tte recipient block image 2 To modify a block layout select tte name of tte desired layout from tte drop down list tten click Edit 3 Follow steps 3 8 in...

Page 43: ...3 Itsertitg a block itto the mait utit 3 In tte classic view of tte recipient block panel block ttumbnail images are refrested eact tme afer you tave inserted a block into a slot First click tte block ttumbnail tten type in a block ID or add a layout In tte new view for recipient blocks tte following optons are available for you to ctoose from afer tte block ttumbnail image is displayed in tte Unu...

Page 44: ... removed from its slot and afer recording its image tte representatve image of tte slot on tte interface is displayed as Empty Warninn Make sure ttat you tave selected a correct layout for tte inserted block so ttat corresponding dimension values bott of tte layout and tte block are identcal For example if Custom block siie opton is selected do not ctoose a layout dimension 24x37 for a block witt ...

Page 45: ... recogniized properly tte following message appears Click Yes and tte Barcode window is opened in wtict you can modify setngs 6 If tte recipient block does not tave any barcode type in tte recipient block ID below tte preview image of tte recipient block 7 Repeat steps 2 and 5 untl you tave typed in all tte block IDs you want to use 8 Click tte sctematc picture of tte frst recipient block you want...

Page 46: ...ered into tte text feld below tte donor block image Afer entering tte donor block ID and titng enter or clicking tte buton tte label area disappears automatcally Click to make tte label area reappear NOTEn If you tave already imported donor block data from a fle tte last imported data is used and also merge is recommended tte list of Donor IDs relevant to tte string appear wtile entering tte ID Se...

Page 47: ...d witt matcting IDs tteir ttumbnails are displayed in a window from wtict you can select one to use it as tte slide image superimposed on tte donor image 5 Afer tte barcodes of donor or recipient blocks are identfed ttey are automatcally displayed bott in tte Recipient Block ID and Donor Block ID felds If barcode reading was not previously set tte Barcode panel of Setngs window appears in wtict yo...

Page 48: ...tte text feld under tte label image If you tck Save to Database you can later view ttis informaton in your export fles Select Unique ID to use tte donor recipient name for barcode informaton NOTEn Barcode reader recogniizes ASCII 32 166 ctaracters only extraneous ctaracters will be deleted from tte string 6 If you want to remove a donor block from tte device click at tte botom rigtt corner of tte ...

Page 49: ...apable of creatng TMA blocks and core sampling into PCR tubes at tte same project or you may as well use any of tte two optons exclusively If you want to use tte PCR sampling functon of TMA Grand Master simply insert tte necessary amount of PCR cassetes into tte designated slots Tte sofware automatcally scans tteir presence and positons during loading block and or cassetes to donor trays Tte PCR b...

Page 50: ...and tte sofware makes it available for you to select some of tte blocks on recipient trays as cleaning blocks To mark a recipient block as a cleaning block click tte icon at tte rigtt side of tte block ttumbnail image You must select tte cleaning block frst tten tte PCR mode can be actvated Wten recipient or cleaning blocks donor blocks and PCR cassetes are inserted tte transfer process can be sta...

Page 51: ...dow opens NOTEn Imane Editor functon is inactve if a block is marked as cleaning block If ttere is no suct buton visible on tte block image go to Settinns Imanes panel tten actvate Imane Editor functon For more informaton see secton 6 Settgs Modify setngs by dragging sliders to tte required directon to create an image ttat is acceptable for you Select tte scope of applicable setngs at tte Apply to...

Page 52: ...corner of eact view Before startng tte transfer process follow tte sequence of steps below to place markers on donor blocks from wtict locatons cores will be extracted to tte selected recipient positons or PCR tubes 1 To place a PCR marker on a donor block frst turn on PCR functon by clicking tte PCR buton on tte rigtt of tte donor block image August 6 2020 Rev 1 3DHISTECH Ltd 52 116 ...

Page 53: ...ou tave not yet entered any TubeID for tte tubes a message appears 3 Enter Tube IDs into tte corresponding felds Hit Enter to accept IDs and click OK to exit from tte PCR Tube ID Editor window 4 Afer you tave entered all tte PCR Tube IDs tte PCR cassete view includes all tte IDs August 6 2020 Rev 1 3DHISTECH Ltd 53 116 ...

Page 54: ...onor block is four 1 5 2 0mm or eigtt 0 6 1 0mm eact PCR tube can contain up to four or eigtt cores of tte corresponding donor block A PCR cassete wten loaded witt 10 PCR tubes is capable of receiving 40 or 80 cores total Important It is available for you to import markers already placed on tte virtual slide 6 If you want to remove an existng marker click it witt tte rigtt mouse buton Tte correspo...

Page 55: ...transferred and clicked Start tte ttumbnail of ttat specifc block will be moved to tte back end of tte block queue NOTEn You can interrupt tte puncting process anytme by clicking tte Stop buton Tte stop functon represented by tte Stop buton in tte applicaton window can be called by pressing a relevant totkey ttat is tte Pause key by default Use ttis key if it is more difcult to interrupt tte proce...

Page 56: ...r tte process is started will be displayed as on tte recipient preview image Upon clicking Start ttey will turn to and processed accordingly You can refrest tte image of tte label and block by pressing tte Refresh buton next to tte block Accordingly tte process state is also displayed on tte blocks represented by gear icons of tte same color as on tte recipient layout image Blue marked but process...

Page 57: ...t block and if you move tte mouse cursor over its spot on tte TMA layout a pop up frame appears Lef click tte spot to make tte frame statc and tte marker positon of tte actual core is displayed in purple on tte donor block image In tte frame tte donor image is displayed by default witt tte informaton ttumbnail image label and donor block data of tte processed core If you tave applied a slide as ov...

Page 58: ...f tte recipient block By moving tte cursor over a core wtere a spot slide is applied a spot slide preview image is displayed Zoom and Save imane are also available For donor blocks wtere slide overlay is applied tte original slide can also be open in eitter CaseViewer for MRXS slides or tte associated program for image slides by clicking on tte Slide Open buton August 6 2020 Rev 1 3DHISTECH Ltd 58...

Page 59: ...Use a layout ttat is entrely flled to make sure ttat tte image stored in tte database is up to date If you would like to leave some cores empty for orientaton purposes use tte Inactve cores feature from tte Layout Editor prior startng to fll tte cores in tte Recipient block 9 If puncting is completed to all tte selected donor blocks remove tte completed donor blocks from tte tray as described in s...

Page 60: ...ck from wtict all of tte selected cores are extracted If ttis tappens you must click tte lock buton frst on tte GUI tten tte buton on tte door to open it Tte lock automatcally turns green afer a process is fnisted Eact tme afer you remove or replace a block tte camera records tte block positon so tte sofware displays an updated image almost instantly Important Make sure wten removing fnisted donor...

Page 61: ... applicaton by clicking Exit If you tave exported data and you want to exit tte applicaton click Yes in tte displayed window Turn tte main unit and tte power supply of Clean tte main unit For more informaton see ctapter 7 2 Cleatitg Tasks 5 6 Slide Overlay Witt tte Slide overlay functon it is available for you to select a slide image from various sources and of diferent formats superimposed on tte...

Page 62: ... to a marker in CaseViewer tte note and tte color of tte marker will be displayed in tte Excel steet in a separate column wten exportng block data NOTEn TMA Control sofware is compatble also witt Pannoramic Viewer but adding notes to TMA Markers is not supported To initate superimpositon follow ttese steps 1 Enter donor block ID and tit Enter key or click 2 Click NOTEn Wten titng Enter tte searct ...

Page 63: ... text boxes for automatc superimpositon To manually locate and import tte image click Browse slide Browse imane or CC Connect NOTEn For tte best matcting results Automatc Overlay functon performs beter wten MRXS fles are searcted for by defning locaton witt tte Browse slide opton At otter images types make sure ttat tte image of tte entre block is captured and saved NOTEn CaseCenter server connect...

Page 64: ...ary To contnue click Next 5 In CaseCenter browse for tte slide in tte folder tree tten lef click on tte slide to import it NOTEn Make sure ttat Open slides in Applicaton opton is selected in CaseCenter 6 If automatc searct tas succeeded and one image is found according to tte specifed ID in one of tte set folders tte image will be applied on tte donor image NOTEn If you modify tte donor ID and you...

Page 65: ...ut if you would like to select tte matcting slide image witt a diferent automatc orientaton click at tte top of tte overlay editor buton 8 Furttermore if automatc slide superimpositon did not provide acceptable result click at tte lef side of tte donor image to open Manual Overlay window August 6 2020 Rev 1 3DHISTECH Ltd 65 116 ...

Page 66: ...ference Points by clicking tte buton so ttat it turns green Place two or ttree reference points on tte block image and refne tteir positons on tte slide image Wten moving tte reference points tte donor slide image is automatcally aligned If reference points are misplaced you can delete all of ttem by clicking tte Remove reference points buton Afer marking reference points tte functons izoom rotate...

Page 67: ...agging You can also modify tte transparency level of tte image layer by moving tte Opacity slider allowing you to set tow muct tte slide image stould obscure tte donor block image Opacity slider tte set value is saved for tte specifc donor is designed to adjust tte amount of superimpositon so ttat tte donor and slide images can aligned more accurately NOTEn Tte fnal positon of tte superimposed sli...

Page 68: ...olding tte Ctrl key wtile placing tte marker it will not be dragged to close spots automatcally By setng tte sufcient amount of transparency of tte image layer marker positoning becomes easier To modify tte opacity level of tte applied image use tte slider on tte lef of tte donor block image Tte diameter of tte marker is set identcal witt tte siize of tte loaded tool Markers become displayed witt ...

Page 69: ...olor ctart of TMA Markers in CaseViewer see below image Color RGB values Red 255 0 0 Yellow 255 255 0 Green 0 128 0 Blue 0 0 255 For otter colors and tteir RGB values please follow ttis link ttp www rapidtables com web color RGBCColor ttm 7 On tte fnal overlayed donor image also tte closed annotatons created in CaseViewer and saved to tte slide appear automatcally Ttese annotatons may be of telp w...

Page 70: ...el or block by pressing tte Refresh buton below tte donor block image Important Tte very last status of tte overlaid slide image is saved for a project NOTEn If a donor block is used in multple projects tten tte actual donor block image is recorded in eact project and separate images are included in tte export fles Also diferent image layers are saved to projects Tte following layers are saved sep...

Page 71: ... tte specifc donor block can be appended to tte database 3 Tte content of tte MedicalInfos data steet can be modifed witt tte Merne or tte Replace functon Tte imported data are stored in tte MedicalInfos data steet ttat can be selected as a record wten exportng Important Column teaders i e frst row in tte table of tte imported Medical Info fle must not contain strings consistng only of numbers Aug...

Page 72: ...can be accessed afer clicking Settinns Click Restore in tte Setngs window to restore tte original data set see ctapter 6 Settgs for more informaton 5 To append new informaton to tte existng data set select Merne so additonal columns will be inserted into tte steet Donor Block ID will become Donor Name no otter data will be overwriten August 6 2020 Rev 1 3DHISTECH Ltd 72 116 ...

Page 73: ...odifed manually but can be saved witt tte Save for Editnn opton Click Save to Database to save imported data 5 8 Restore Medical Informaton Database Tte program makes it available for you to restore a medical informaton database tterefore all tte data during tandling tte project before exportng can be omited For more informaton on tow to restore a database see ctapter 6 Settgs August 6 2020 Rev 1 ...

Page 74: ...MA Register from TMA Control and you are not logged in to tte CaseCenter server you can log in ttrougt tte TMA Renister window 5 9 2 Settinns Click tte TMA Register Settinns buton to open tte applicaton setngs menu You can set TMA Slides Theme and Lannuane optons tere August 6 2020 Rev 1 3DHISTECH Ltd 74 116 ...

Page 75: ...see ctapter 6 Settgs Creatnn irtual or Local Spot Slides To create virtual or local spot slides follow tte steps below 1 Select tte folder to Set CaseCenter slide folders for TMA slides 2 If you want your spot slides as virtual slides cteck Create irtual Spot Slides to CaseCenter 3 Select tte folder wtere you want to place tte generated folder structure for tte virtual slides Select a folder witt ...

Page 76: ...u set your TMA slides folder precidely in step 1 tte links to tte TMA slides appear in tte Slide Link column 11 Save tte table again 12 Click tte Start Spot Slide Separatnn buton next to tte Search TMA Slides in CC buton 13 Tte progress informaton is displayed on tte top of tte Details panel 14 Tte local and tte virtual slides are stored in tte folders you set in Settinns menu August 6 2020 Rev 1 ...

Page 77: ... 200 recipients ttey will be placed in tte Reiipients2 folder In tte Reiipients group folder you can fnd tte recipients folder witt tte same name as your recipient Ttis folder will contain virtual slides from all TMA slides Under ttis folder you can fnd tte Layout folder wtict subfolders will represent your layout Tte created virtual spot slides can be found in tte SpotSlides folder in Row Column ...

Page 78: ...steets will be created wittin tte fle for eact block If you want to fll otter sample cores into tte blocks at anotter tme ttese fles can be used and to wtict donor block data can be appended Depending on tte mode you operate TMA Grand Master in eitter mixed mode or TMA only or PCR mode only tte Export Window contains diferent felds and optons for export setngs 1 In mixed or TMA mode afer tte TMA b...

Page 79: ...a new scteme frst click tten you can custom defne a record set from tte SQL database of tte related project NOTEn Make sure to select appropriate felds wten creatng a new scteme Database records are freely selectable and erasable from tte set Tte MedicalInfos record is empty by default if ttere is no data imported yet NOTEn previously imported clinical data can be exported togetter witt TMA datase...

Page 80: ...n You must tave an actve CaseCenter connecton to save overlay link data Wittout it tte fle is exported wittout tte overlay link data NOTEn Open fle witt tte appropriate sofware for example use Excel to open fle in XLS or XLSX or OpenOfce Calc to open fle in ODS format 4 Click Save to create tte output fle or Cancel to exit tte window wittout saving Tte generated spreadsteet contains four worksteet...

Page 81: ...olumns tte image of tte block is displayed in tte web browser applicaton set as default recommended HTML browsers are Firefox and Ctrome For Donor images tte four aforementoned layers can be viewed separately II RB_1 steet contains recipient block related data table wtere tte core IDs are stown according to tteir positons in tte layout III RB_1_Donor steet contains recipient block related data tab...

Page 82: ...t Project name Creaton date Modifcaton date Diameter Layout name Layout siize row x column Number of Donor Number of Core Clone Group Recipient block image Tip If you want to import tte exported data to TMA Module you need to create a worksteet named to Slides including stain types recipient block IDs and slide names August 6 2020 Rev 1 3DHISTECH Ltd 82 116 ...

Page 83: ...s Note ttat tte PCR Tube IDs column is included on tte steets only if you tave added IDs to tte PCR tubes tte PCR Tube ID functon is enabled in tte Setngs and tte fnal export fle contains tte Annotaton name column only if ttere is an annotaton on tte digital slide you tave superimposed as an overlay on tte donor block and from wtict a core or cores tave been extracted in your project On tte By_Don...

Page 84: ...pot slides only if tte Spot slide functon was actvated and ttus an image of tte extracted spot is saved to a designated folder Upon clicking a spot image link tte spot slide opens in CaseViewer applicaton B If you tave selected tte Free PCR sampling mode tte Excel fle contains ttree worksteets On tte By_Donor_Block steet tte data are sorted per donor PCR tube and tte steet includes columns suct as...

Page 85: ...arked witt red annotaton 2 are marked witt yellow and 1 is marked witt green tten 3 separate rows are presented in ttis steet sorted by annotaton colors On tte By_Sample_Core steet you can fnd PCR data sorted by tube in tte following columns Donor Block ID Donor Block Images Donor Core Heigtt Spot Slide PCR Tube ID Tube e g 2 4 PCR Tube 7 Annotaton Color and Annotaton Name NOTEn Spot slide column ...

Page 86: ...maton database Note ttat tte columns can vary depending on tte imported data type By_Annotaton_Color steet lists data by cores but data are sorted by annotaton color per donor Ttis steet is created only if at least one annotaton color is present in tte export parameter list Ttis steet is appended witt tte Annotaton Color column containing annotaton colors For example if 6 cores tave been extracted...

Page 87: ...Medical Informaton data NOTEn Medical Informaton data only appears if it is previously imported from a separate fle for example Excel in Import Data see ctapter 5 7 In tte Date secton tte date of tte frst created project is applied as startng date In tte Search secton Project name Recipient name Donor name and Diameter preferences can be defned During entering a text string in any of tte felds cor...

Page 88: ...ear on tte list at tte rigtt side of tte window Table columns can be reordered by dragging and tteir order is reversible by clicking tte teader Searcting by Recipient Name tte available parameters are tte following Recipient Name Layout Siize Rows x Columns Layout Name Diameter Recipients Heigtt Project Name Modifcaton Date Creaton Date Creaton Date UTC Modifcaton Date UTC Number of Recipient Core...

Page 89: ...city select Annotatons to view annotatons of tte slide image and select Markers to view TMA markers placed on tte donor block To save image to tte designated downloads folder of tte browser click tte buton Search Result table is expandable witt otter columns afer clicking and selectng tte desired preferences Here tte order of columns displayed in tte table can be set as well by dragging teader nam...

Page 90: ...s are available by clicking in tte top rigtt corner of tte TMA Register window For Auto TMA Slide Search in CaseCenter and Automatc Spot Slide Separatnn setngs including Creaton of irtual Spot Slides to CaseCenter see ctapter 5 9 2 Settgs It is available for you to flter Recipient blocks based on date project name and or Recipient block name Ttere are two separate mettod for uploading TMA Slide da...

Page 91: ... propertes secton contains several data suct as Layout name Layout siize Rows x Columns Number of cores Number of donor blocks Diameter Creaton date In Slide view frst enter TMA Slide data tten select multple Recipient blocks to be linked witt based on flter setngs In bott views use to select all flter list items and click to select none August 6 2020 Rev 1 3DHISTECH Ltd 91 116 ...

Page 92: ...mn or select an existng column from tte list Reorder column entries NOTEn If you add a new column to tte table and you defne tte teader manually afer entering a data into tte column felds tte TMA Slide Informaton secton appears on tte Search panel Column teaders can be saved as a scteme and ttis scteme can be reused anotter tme Click to save table as a scteme In tte Scteme Directory window wten ty...

Page 93: ... of Blocks Wten you close a project tte sofware ofers you to remove all tte blocks donor and recipient from tte device in one session Afer clicking Yes frst open tte door remove blocks tten close tte door Once tte door is closed and tte drum turns to tte next tray positon repeat procedure untl all tte blocks tave been taken out from tte device If all tte blocks tave been removed tte sofware closes...

Page 94: ...folders you tave set under tte sectons Set local Slide folders or Set local imane folders Actvate Auto Search In CaseCenter to add results for Overlay functon based on Folder Case Block and or Slide criteria Type in or browse for folder patts for automatc searct eitter for Slides or images Folders ttat tave been set can be displayed afer clicking tte down arrow and afer you tave selected an item f...

Page 95: ...t tte siize of tte actual tool inserted is selected by default Actvate Show closed annotatons from slide opton to allow tte visualiizaton of annotatons of tte slide NOTEn Tte export fle includes marker annotaton data suct as name color and comment Restore Medical Info Database On tte Restore Medical Info Database panel select tte database ttat you want to restore tten click Restore Tte database tt...

Page 96: ...justment migtt be necessary to perform an accurate core removal depending on tte locaton of tte tssue wittin tte block Value ranges from 500μm to 13000μm Warninn If tte tssue specimen stcks to tte base plate of tte cassete so ttat tte puncter cannot take out tte core entrely or properly you may specify a negatve value at tte Punchinn depth μm feld Bear in mind ttat tte puncter needle stould be mov...

Page 97: ...slide in CaseViewer or tte associated program for image slides by clicking on ttis buton Imane Zoom Save Block imane Imane Editor Witt tte Save Spot Slides automatcally opton a collecton of images of marker annotatons placed on tte digital slide locatons of cores extracted from tte donor block a so called spot slide in MRXS format is saved automatcally if enabled By enabling ttis functon tte siize...

Page 98: ...play labels in layout editor and on tte recipient block image Labelinn variatons Click to switct between tte following predefned labeling variatons for rows columns 0 1 2 3 1 2 3 a b c Labeling directon increasing decreasing can be set for rows and columns separately by clicking Labelinn positon Select one of tte positoning modes Alinn to block ednes or Alinn to core positon August 6 2020 Rev 1 3D...

Page 99: ...ere is no project open PCR Inserton Mode Here you can select Conventonal PCR core inserton or Freely selectable PCR core inserton modes Conventonal and free inserton modes are represented in tte GUI by tte following variatons of butons PCR Tube ID If you want to add unique names to tte PCR Tubes select Yes August 6 2020 Rev 1 3DHISTECH Ltd 99 116 ...

Page 100: ...ill be identfed during creatng a project Barcode informaton can be used as an extra informaton instead of donor recipient name If you want to use ttis feature select Custom Infomaton in tte Barcode label content type secton In ttis case barcode informaton is displayed in tte text feld under tte label image If you tck Save to Database you can later view ttis informaton in your export fles Select Un...

Page 101: ...onally cannot read Data Matrix barcodes Once tte Matrox opton is selected set tte type 2D 1D 1D CDV and tte subtype of tte specifc group from tte drop down list Theme Tte graptic user interface now tas diferent types of visualiizaton skins select a preferred skin type by clicking tte arrows lef of rigtt Tte elements according to tte selected skin type are displayed in tte Visual experience secton ...

Page 102: ...TMA Grand Master 3 2 User s Guide 6 Settings Lannuane Tte language of tte sofware can be selected from tte list August 6 2020 Rev 1 3DHISTECH Ltd 102 116 ...

Page 103: ...gttly moistened piece of clott You may also use detergent Wipe of oil or grease contaminaton traces from tte surface of tte main unit witt a coton swab dipped into acetone but keep away acetone ettanol from mirrors and lenses It is forbidden to clean mirrors and lenses witt ettanol acetone Tte user stould prevent any kind of fungal contaminaton on tte opto mectanical parts of tte TMA Grand Master ...

Page 104: ...st or objects dropped in To remove tte door of tte service compartment do tte following 1 Switct of tte TMA Grand Master device 2 Pull out tte trast container 1 Figure 13 Trash container 3 Release lock by pulling out tte lever 1 at tte inner panel and move it to tte marked positon 2 Figure 14 Opening the service compartment door 4 Grab tte tandles and open tte compartment door 5 Clean tte compartm...

Page 105: ...cable 2 Tte fuse compartment is located next to tte main power supply switct at tte back side of tte TMA Grand Master 3 Release lock 1 by pusting tte lever to tte rigtt so tte insert 2 can be pulled out Figure 15 Releasing the lock of the fuse compartment 4 Remove insert from tte compartment Figure 16 Removing the fuse compartment August 6 2020 Rev 1 3DHISTECH Ltd 105 116 ...

Page 106: ...A 250V fuses only 7 4 Contaminated Tray or Touch Sensor Surface If during initaliizaton afer switcting on tte TMA Grand Master device tte program detects possible contaminaton at tte surface of tte F donor block tray between tte positons F9 and F10 or of tte touct sensor tte following message appears on tte screen Click Yes tten follow tte instructons regarding cleaning tte tray surface August 6 2...

Page 107: ...ensor must be cleansed as well According to tte instructons in tte following message by using a dry and clean clott gently rub tte botom surface of tte touct sensor sensor is marked witt red in Finure 18 Figure 18 Touch sensor Afer you fnisted witt tte cleaning tasks you receive tte following message August 6 2020 Rev 1 3DHISTECH Ltd 107 116 ...

Page 108: ...ttamitated Tray or Touch Setsor Surface Block Imane in the Applicaton is of Poor Quality Causen Imaging errors What to don Contact 3DHISTECH support Puncher Falls Out Durinn Punchinn Causen Tte puncter needle is detacted from tte collar What to don Use anotter puncter The Puncher Does Not Pick Up the Core Causen a Tte extracted tssue sample is placed too deep into tte parafn it touctes tte tead of...

Page 109: ...indows 7 afer tte update process is fnisted Follow tte below steps to avoid tte automatc reboot of your PC afer a major update via Windows Update Go to Start menu Control panel Windows Update Channe settinns Select Download updates but let me choose whether to install them item from tte Important updates drop down list Click OK to accept ctanges and close tte window August 6 2020 Rev 1 3DHISTECH L...

Page 110: ... recommended to use Defer feature upnrade opton ttat can delay feature updates and security updates will be available only HDD is inaccessible not writable Tte System Administrator must ensure accessibility by tte control sofware to some folders on tte computer For instance write access must be granted to C ProgramData 3DHISTECH TMAControl folder its sub folders fles and in additon enable tte sofw...

Page 111: ... GTMA sofware applicaton If tte problem stll persists try to reinstall it Camera Error messane is received Causen Camera cables are not connected properly Solutonn Follow instructons in tte message box Important Tte folder in wtict log fles are contained is C ProgramData 3DHISTECH TMAControl_LOG Log fles can be updated by running tte log_refresh bat fle August 6 2020 Rev 1 3DHISTECH Ltd 111 116 ...

Page 112: ...deptt of tte block is 13 5 mm Tte smaller siize 7 5 mm in deptt means tte parafn ttickness from tte upper edge of tte plastc cassete Tte teigtt of parafn blocks in parentteses means tteir total deptt togetter witt tte plastc cassete Warninn Do not use parafn blocks wtict dimension values are tigter ttan 24mm W x 37mm H x 10mm D otterwise tte drilling and puncting teads may be damaged If tte parafn...

Page 113: ... 512 44 352 Max 3 3 approximate values 10 2 Accessories Accessory Dimensions in mm Number of items Base mold for parafn blocks 37 24 7 5 3 Drill bit Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 2 2 2 2 Puncter Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 3 3 3 3 Puncter ctuck and Drill bit ctuck N A 2 Piston Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 1 1 1 1 For Drill bits Puncters and Pistons ttere are four distnctve color markings for eact diferent siizes...

Page 114: ...illion Pixel 10 4 Ambient Conditons Conditon Suitable Ranne Shipment packed Ambient temperature 40 C to 70 C Storane Ambient temperature 10 C to 40 C Maximum relatve air tumidity 75 at 35 C Operaton Ambient temperature 10 C to 40 C Maximum relatve air tumidity 75 at 35 C Maximum alttude 2000 m Air pressure range 500 tPa to 1060 tPa Degree of polluton 2 August 6 2020 Rev 1 3DHISTECH Ltd 114 116 ...

Page 115: ...IP 20 Electrical safety in compliance witt EN 61010 1 IEC 61010 1 and taking CSA UL regulatons into account Over voltane catenory II Radio interference suppression in accordance witt EN 55011 class B Noise immunity in accordance witt EN 61326 2 6 2005 Main unit line input voltane 24 V DC 1 V Mains supply voltane 90 240V Mains frequency 50 60 Hiz Power ratnn 300W August 6 2020 Rev 1 3DHISTECH Ltd 1...

Page 116: ...38 40 41 42 43 50 51 70 M MRXS 8 P PCR 8 9 28 29 35 44 47 48 50 51 54 Punct 9 12 27 28 29 30 31 33 34 35 36 37 45 51 53 92 96 97 R Recipient block 8 9 28 29 30 31 35 36 41 42 43 44 48 50 51 53 67 69 70 71 87 Researct project 9 S Slide 4 8 30 35 50 55 57 58 59 60 71 T TMA 8 9 13 60 69 71 TMA Control 15 17 18 19 20 28 31 42 55 TMA Grand Master 6 9 10 11 12 13 20 31 35 36 37 42 47 48 51 55 87 88 89 9...

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