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ZEISS
5 First Operating Steps | 5.5 Modifying Gun Parameters and Optibeam Mode
5. In the
Stigmation
navigation box, use the
scroll bars or the red marker to obtain the
sharpest possible image.
INFO:
The specimen detail should just be pul-
sating without shifting.
INFO:
To obtain optimum results, alternately
correct focus and astigmatism.
6. To deactivate the reduced raster, in the
Toolbar
, click the REDUCE icon.
5.4.10.7 Adjusting Final Magnification, Contrast and Brightness
Procedure
1. Reduce the magnification to the required value (e.g. 25,000 x).
2. To activate auto contrast and brightness, click the
LEVELS
icon in the
Toolbar
.
5.4.11 Saving the Image
Procedure
1. Once a steady contrast and brightness level is reached, click the
PHOTO
icon in the
Tool-
bar
.
INFO:
This runs a macro that automatically changes the scan speed to 8, the noise reduc-
tion to line integration with 4 lines and freezes the image at the end of the frame.
à
A red dot at the right bottom of the image area indicates that the image is frozen.
2. From the
Menu Bar
, select
File > Save Image
.
à
The
Export TIFF
dialog is displayed.
3. To change the save path, click
Change Directory
.
à
A file explorer window is displayed.
4. To confirm the selected path, click
Select Folder
.
5. Enter the file name in the
Filename
input field.
6. Click
Save <file name>.tif
.
7. To continue imaging, middle-click the
PHOTO
icon.
5.5 Modifying Gun Parameters and Optibeam Mode
5.5.1 Using the Automatic Gun Alignment Functions
Automatic gun align is used to automatically align the shift and/or the tilt of the beam, to the cen-
ter of the column.
Two automatic gun align functions are available:
Instruction Manual ZEISS EVO | en-US | Rev. 10 | 354706-0780-006
79
Содержание EVO
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