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ScreenWorks Software Overview
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Correction
Use the Correction dialog to apply data-correction algorithms, and to
view ratiometric data. These changes affect:
• Data displayed in the Multi-Well Graph and Detail Graph .
• Data displayed in group statistic table and graphs.
• Data in files are exported manually or automatically (see
Exporting Data on page 99 ).
• Data in printed output configured in the Settings process page
(see Auto Print Options on page 78 ).
Plainly, once data are exported, they cannot be changed, so settings
made in this dialog in protocol files are important for these output
options. For viewing data in ScreenWorks Software, however, since raw
data are always kept, correction options selected in protocol files can
be altered when the data are viewed in resulting data files.
The table below provides brief descriptions of the options in this dialog
box; see Appendix B: Data Processing Algorithms on page 273 for a full
description.
Item Description
Crosstalk
Correction
Only available with Aequorin, ICCD camera. Check this
option to correct all wells for crosstalk from neighboring
wells. The crosstalk function is based on well-to-well
distance and the measured percentage of crosstalk.
Positive Control
Scaling
Check to average each maximum RLU value from the
positive control group and normalize all samples to this
value (set at 100%). This function is useful when graphing a
dose-response curve or when comparing data between
experiments.
Negative
Control
Correction
Check this option to have an average negative control well
value and ratio-to-well value calculated for each sample
interval (time point). This ratio is calculated for each sample
interval and applied to all wells. This function provides a
good correction for signal drift and artifacts.