FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H
277
Determining Negative Control Correction
The negative control correction factor is derived by first calculating the
mean fluorescence of each of the samples of the negative control wells.
The mean of Sample 1 is divided by the mean of each of the samples to
give the sample-specific correction factor.
Each sample is multiplied by its sample-specific correction factor.
For example, A1 Sample 1 is multiplied by 1.00.
Sample
Time
Well
A1
– Ctrl
A2
– Ctrl
A3
– Ctrl
Mean
Correction
Factor
1
0
8960
8925
8930
8938
1.00
2
5
9194
9030
8836
9017
0.99
3
10
9408
8925
8648
8994
0.99
4
20
9632
8820
8648
9033
0.99
5
25
9632
9240
8648
9173
0.97
6
30
9856
8925
8930
9237
0.997
Sample Time
Well
A1
– Ctrl
A2
– Ctrl
A3
– Ctrl
A4
Exp
A5
Exp
A6
Exp
A7
+ Ctrl
A8
+ Ctrl
A9
+ Ctrl
1
0
8960
8925
8930
8938
8924
8976
8900
8930
8961
2
5
9194
8940
8748
8957
8643
9088
8370
9027
9177
3
10
9314
8836
8562
9172
8643
9189
8547
9027
9585
4
20
9536
8732
8562
48560
48015
42412
50223
48391
55064
5
25
9343
8963
8389
39120
39518
34629
45755
45590
49955
6
30
9560
8657
8662
31719
32932
28693
43165
46502
49955
Note:
The negative control correction factor wells contain the same
cells and dyes as the experimental and positive control wells.
Содержание FLIPR Tetra
Страница 1: ...FLIPR Tetra High Throughput Cellular Screening System User Guide 0112 0109 H December 2011...
Страница 12: ...Contents 12 0112 0109 H...
Страница 16: ...System Overview 16 0112 0109 H...
Страница 40: ...System Hardware Features 40 0112 0109 H...
Страница 148: ...Exchanging Hardware 148 0112 0109 H...
Страница 156: ...Calibration and Signal Test 156 0112 0109 H...
Страница 196: ...Running an Experiment 196 0112 0109 H...
Страница 232: ...Robotic Integration 232 0112 0109 H The following drawings illustrate these requirements...
Страница 282: ...Data Processing Algorithms 282 0112 0109 H...
Страница 294: ...Consumables and Accessories 294 0112 0109 H...
Страница 298: ...Using AquaMax Sterilant 298 0112 0109 H...
Страница 302: ...Electromagnetic Compatibility EMC 302 0112 0109 H...