Molecular Devices FLIPR Tetra, Руководство пользователя

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7
Calibration and Signal Test
Chapter 7 provides the procedures and information required to
calibrate the optics and run a signal test of the FLIPR
®
Tetra System.
Optical Calibration
Calibrating the optics of FLIPR
®
Tetra System requires taking an image
of the 96-, 384-or 1536-well plate and using it as a mask to locate the
wells. Typically, calibrations are performed with the following
procedures:
After LED banks or pipettor head has been exchanged.
After a new plate has been added to the plate library.
If no filters are in the instrument, install them at this time (see
Changing Filters on page 141 ).
Adding a Read Plate to Plate Library
The FLIPR
®
Tetra System has default plate masks for 96-, 384-, and
1536-well read plate types. If you want to use a plate type that varies
significantly from the default formats, you must create new dimensions
and mask for that plate type. Use the description of the Plate Library
menu item, (see Plate Process Definition on page 62 ), as a reference
guide for options and parameters available when adding a plate to the
software.
To add a plate and mask to the software:
1. Prepare a 1 x 10
-8
M fluorescein solution.
2. Ensure the microplate bottom is clean and free of scratches and
dust.
3. Dispense enough fluorescein solution into each well so as to
cover the bottom. Dispensing half of the total well volume is
typically a safe volume with which to work. This may be done
using FLIPR
®
Tetra System pipettor.
4. Place the microplate containing fluorescein in the read position
and select Plate Library from the Tools menu.
5. From the Plate Process Definition list, select a plate with a
similar well format.
6. Click Copy Plate .