Molecular Devices FLIPR Tetra, Руководство пользователя

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FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 111
Edit Aspirate
(continued)
Drain Reservoir Speed
1
— Select the speed with which to
drain the cells from Cell Reservoir to drain destination.
Drain Destination
1
— Select a destination where cells should
be drained from Cell Reservoir after pipettor aspiration.
Height— Enter distance (measured in μ L) from the bottom of
the well the tips should start aspirating.
Note: Tips move downward full volume of aspiration,
for example, aspirate 10 μ L with a start height of 35
μ L will put the pipettor height at 25 μ L after
aspiration. Pipettor head movement helps prevent
well overflow when tips are submerged in the well. If
downward pipettor motion is not desired, set tip
height to minimum value. Tip movement downward
halts when the tips reach 1/30
th
well volume.
Hold Volume —Enter size of air gap (in μ L) following
aspiration. Air gaps prevent liquid from leaking out of the tip
before dispensing.
Removal Speed —Enter rate (in mm/s) at which tips are
pulled from well immediately after aspiration. This value is
adjusted depending on fluid viscosity or volume of fluid in the
well.
Note: For low volume transfers, a slow up-speed may
improve precision.
Put Tips in Well
Before Read
Check this option to place tips in the read plate prior to
beginning read intervals.
This option is suggested for assays in which placing tips
below the fluid surface can prevent excessive turbulence.
Enabling this option helps ensure accurate timing of fluid
dispense relative to reads.
1. These options are only available when Cell Reservoir is installed and selected in
Source Plate 3 settings.
Item Description