FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H
177
Precipitation in the Reagent Buffer
The FLIPR
®
Calcium Assay Kit is compatible with numerous buffers. Use
buffers shown to work in previously established assays, if available.
Response is Smaller than Expected
The agonist and antagonist may stick to the tips and trays. Use up to
1% BSA in all compound buffer diluents and presoak tips in compound
buffer with up to 1% BSA.
Apparent Well-to-Well Variation is Observed
AquaMax DW4 or equivalent dispenser is recommended for use with
additions of cells or dye prior to the assay on the FLIPR
®
Tetra System if
apparent well-to-well variation is observed. In some cases allowing the
plates to stand at room temperature prior to use or adding a single mix
cycle in the compound or assay plate may decrease well-to-well
variation.
FLIPR Membrane Potential Assay Kit Protocol
This section provides the following information and procedures:
•
Materials required for the assay
•
Cell preparation guidelines
•
Dye loading procedure
•
Troubleshooting tips for membrane potential assays
Required Materials
The following materials are required for running a membrane potential
assay:
Note:
Do not use the same source plate for presoaking and compound
addition when using a 384 Pipettor head in the FLIPR
®
Tetra System.
Instead, use a 'Boat' for the presoak.
Item
Source
FLIPR
®
Tetra System with Membrane Potential
Optics Kit installed:
FLIPR
®
Tetra System LED Module 510–545 nm
FLIPR
®
Tetra System Emission Filter 565–625
nm
Molecular Devices
Содержание FLIPR Tetra
Страница 1: ...FLIPR Tetra High Throughput Cellular Screening System User Guide 0112 0109 H December 2011...
Страница 12: ...Contents 12 0112 0109 H...
Страница 16: ...System Overview 16 0112 0109 H...
Страница 40: ...System Hardware Features 40 0112 0109 H...
Страница 148: ...Exchanging Hardware 148 0112 0109 H...
Страница 156: ...Calibration and Signal Test 156 0112 0109 H...
Страница 196: ...Running an Experiment 196 0112 0109 H...
Страница 232: ...Robotic Integration 232 0112 0109 H The following drawings illustrate these requirements...
Страница 282: ...Data Processing Algorithms 282 0112 0109 H...
Страница 294: ...Consumables and Accessories 294 0112 0109 H...
Страница 298: ...Using AquaMax Sterilant 298 0112 0109 H...
Страница 302: ...Electromagnetic Compatibility EMC 302 0112 0109 H...