Molecular Devices FLIPR Tetra, Руководство пользователя

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FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 89
The table below describes the settings and options in the Grouping
dialog box:
Item Description
Groups Displays the list of defined groups, including group
name (typically compound name), concentration and
notes associated with the group. Wells assigned to a
group in the grid below have the same color as the
group in the list.
Positive Controls —Use this group to assign positive
control wells, typically the maximum response to a
concentration of an agonist.
Note: Positive Control wells need to be
defined in order to use the Positive Control
Scaling Correction .
Negative Controls —Use this group to assign negative
control wells, typically buffer addition controls.
Note: Negative control wells need to be defined
in order to use the Negative Control
Correction feature.
BF Controls —Use this group to assign wells in which
to measure background fluorescence. Measurements
for each time point from these wells are used for
Background Fluorescence Correction for ratiometric
data (see Ratiometric Options on page 94 ).
_add new group— Use this to define a new individual
group or a series of groups that are user-defined.
Add/Edit Group Opens the Edit Group dialog to edit the group
selected in the Groups list. This could be an existing
group, or create a new group by selecting the Add
New Group row.
Shortcut: Open the Edit Group dialog by
double-clicking on a group in the Groups list.
Delete Group Deletes the group selected in the Groups list.
Any wells assigned to the group lose their coloring,
indicating that they no longer belong to any group.