Molecular Devices FLIPR Tetra, Руководство пользователя

Страница: 295 / 302

0112-0109 H 295
D
Using AquaMax Sterilant
AquaMax Sterilant can be used to clean and sterilize the fluid paths in
the Cell Reservoir and Cell Suspension module.
Principle of Use
Accumulated residues and contaminating microorganisms have the
potential for causing significant adverse effects on the operation of
liquid handling equipment. Proteins in cell culture media, salts and
minerals in buffers and other reagent components used in liquid
handling equipment can build up on fluid path surfaces, clogging
channels and probes resulting in incomplete or inaccurate dispensing
and aspiration of liquids. In addition, microorganisms such as bacteria,
fungi and molds can also accumulate in the fluid path creating similar
fouling problems. Simply flushing the fluid path with water or
ethanol/water solutions is often ineffective in the removal of residue.
AquaMax Sterilant was developed for the purpose of removing organic
and inorganic material, and killing microorganisms, including
endospores, in liquid handling instrument fluid paths. In addition,
AquaMax simultaneously removes endotoxin from the fluid paths.
Endotoxin is a substance produced by Gram negative bacteria that is
known to cause a variety of significant cellular reactions in vitro,
including cell activation and death. Thus minimizing the amount of
endotoxin in fluid paths is necessary in order to avoid detachment and
adverse effects on mammalian cells during the course of subsequent
experiments. A two-hour treatment with AquaMax will substantially
remove endotoxin adhering to interior fluid path surfaces.
Contents
• 4X Concentrated Solution A: detergent in buffered aqueous
solution (2 x 1 L bottles).
• Solution B: sodium hypochlorite in aqueous solution (2 x 14 mL
bottles) [16 500 mL DW4 washes].
Materials Required but Not Provided
• Deionized water
• 250 mL graduated cylinder
• 1 L beaker, bottle or flask
• 2 mL pipettor