Molecular Devices FLIPR Tetra, Руководство пользователя

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FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 109
Aspirate Table (Standard Pipettor)
The following table describes configuration options for the upper,
Aspirate , section on the Transfer Fluid process page when a
standard pipettor is selected.
Item Description
Fluid Transfer
Type
Select a type of fluid transfer:
Single Aspirate–Single Dispense —Aspirates one liquid
volume and dispenses that entire liquid volume in one action
into a plate.
Single Aspirate–Multiple Dispense —Aspirates one liquid
volume and dispenses up to four distinct liquid volumes into
four distinct quadrants or plates. Add additional dispenses by
making the dispense volume less than the aspirate volume.
Note: The last dispense expels all liquid remaining in
the tip. Tips to improve Single Aspirate–Multiple
Dispense (by quadrature) pipetting precision include:

When pipetting do not use hold and expel volumes.
In addition use the slowest removal speed (2 mm/s).

Aspirate 5–10% more than is needed from the
source plate (
for example
, aspirate 110 μ L when
dispensing 25 μ L into four quadrants.

Include a mix step with 3 strokes in the desired
source plate to pre-wet tips. Mixing of fluid in the
source plate primes the internal walls of the tips,
reducing surface tension between the fluid and tip
plastic.
Multiple Aspirate–Single Dispense —Aspirates up to four
liquid volumes from up to four aspirate plates and dispenses
entire tip contents into a single plate. To add additional
aspirates, make the aspirate volume less than the dispense
volume.
Read Check this box to link a Read process to the Transfer Fluid
process.
This automatically adds a
Read with TF step to the protocol,
and opens the configuration page for that step.
See Read Process on page 120 for more information about
read process options.
Note: This option is required if you intend to read a
plate while transferring liquid during a kinetic cell-
based assay (for example, calcium mobilization).