Molecular Devices FLIPR Tetra, Руководство пользователя

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Running an Experiment
182 0112-0109 H
Recommended Setup Parameters
Recommended experimental setup parameters are provided below.
Note that the addition speeds are faster than in the conventional
protocol because of the increased robustness of the cells after the new
loading procedure.
Faster addition speeds can lead to better mixing of compounds and
lower signal variance across the plate.
Experimental Setup Parameters
Detection Parameters
EMCCD
Camera
ICCD Camera
Read Mode Fluorescence Fluorescence
Excitation Wavelength 510–545 nm 510–545 nm
Emission Wavelength 565–625 nm 565–625 nm
Excitation Intensity 50
1
% 50
1
%
Camera Gain 50 2000
Gate Open — 6
1
%
Exposure Length 0.4
1
s
1. Can be adjusted to increase or decrease the signal if low RFUs or
saturation problems occur.
0.53
1
%
Read Interval 1 s 1 s
Addition
Parameters
96-Well
Plate
384-Well
Plate
1536-Well
Plate
Compound
Addition Volume
( μ L)
50 12.5 1
Compound
Concentration
(Fold)
5X 5X X
Dispense Speed
( μ L/s) Adherent
Cells
50–100 10–50 5–10
Dispense Speed
( μ L/s) Non-
adherent Cells
10–50 5-20 1-6