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8
Running an Experiment
Overview
This chapter provides a starting point for setting up and running a
kinetic cell-based assay on the FLIPR
®
Tetra System. Running an assay
requires performing the following tasks:
•
Preparing the cells
•
Powering-up the system
•
Running a system check
•
Dye-loading the cells
•
Preparing source plates
•
Setting up an assay protocol
These tasks are explained in detail in the remainder of this chapter.
Following the general procedures for running a FLIPR
®
Tetra System
experiment, protocols are outlined for four types of assays:
•
Fluorescence-based calcium mobilization
•
Fluorescence-based membrane potential
•
Fluorescence ratiometric membrane potential using Voltage
Sensor Probes
•
Luminescence-based detection of calcium mobilization
After the presentation of the assay protocols, guidelines for assay
optimization are provided.
Preparing Cells for Adherent Assays
Location of Cells in the Plate
The excellent signal-to-background noise ratio of the FLIPR Tetra
System is made possible by the instrument’s bottom illumination and
detection system. The read plate is illuminated by the LED modules at
an angle approximately 45. It is recommended to have cells located at
the bottom of the wells during an experiment. Adherent cells are
typically grown overnight in the cell plate, whereas non-adherent cells
are seeded either the night before, on a coated plate, or the day of an
experiment and then centrifuged.
Содержание FLIPR Tetra
Страница 1: ...FLIPR Tetra High Throughput Cellular Screening System User Guide 0112 0109 H December 2011...
Страница 12: ...Contents 12 0112 0109 H...
Страница 16: ...System Overview 16 0112 0109 H...
Страница 40: ...System Hardware Features 40 0112 0109 H...
Страница 148: ...Exchanging Hardware 148 0112 0109 H...
Страница 156: ...Calibration and Signal Test 156 0112 0109 H...
Страница 196: ...Running an Experiment 196 0112 0109 H...
Страница 232: ...Robotic Integration 232 0112 0109 H The following drawings illustrate these requirements...
Страница 282: ...Data Processing Algorithms 282 0112 0109 H...
Страница 294: ...Consumables and Accessories 294 0112 0109 H...
Страница 298: ...Using AquaMax Sterilant 298 0112 0109 H...
Страница 302: ...Electromagnetic Compatibility EMC 302 0112 0109 H...