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K-MINC-1000 Instructions for Use
English
6.3.1 Temperature
Set each chamber temperature to 37°C. Place a NUNC® four-well dish into the front inner corner of each chamber.
Fill the front inner well of each dish with 0.8 mL of media culture and thermally equilibrate.
To measure the temperature, immerse the tips of calibrated thermocouples into the culture media as shown below,
ensuring that the each thermocouple tip sits on the bottom of each well. Close the chamber lid and allow enough
time for the thermocouples to thermally equilibrate.
1. Left chamber thermocouple well position
2. Right chamber thermocouple well position
3. Front of MINC
The temperatures measured will be the user set temperature ±0.2°C.
6.3.2 Gas flow rate
To test flow rate, use an air calibrated gas flow meter connected to the Luer lock outlet beneath the bacterial filter
as shown below.
The gas flow meter should have no further
1. Luer Lock Outlet
2. Flow Meter
restriction on the outlet and be open to atmospheric pressure. Start the
gas flowing. The gas flow meter should indicate twice the user set point value ± 15%. Twice the user set point is
observed because the display shows gas flow rate per chamber.
At each of the following set points, check that the measured flow rate is within the corresponding
acceptable range:
Flow Rate Set Point (mL/min)
Measured Flow Rate Range
(mL/min)
15
25.5 to 34.5
20
34 to 46
25
42.5 to 57.5
If measurements lie outside the acceptable ranges then contact your Cook Medical representative. Gas flow rates
for the MINC are calibrated at 22°C at sea level.
Temperature and atmospheric pressure must be taken into account when calculating the expected gas flow. The
table below shows the flow obtained with altitude when the MINC has been set at 15 mL/min.
IMPORTANT NOTE:
This
functionality testing must be
performed every six months.
2
1
Metres above sea level
Flo
w (mL/min)
Flow variation vs altitude
1
2
3
1
