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K-MINC-1000 Instructions for Use
English
3.6 Device placement
The MINC should be placed on a level secure surface, away from heaters, coolers, air-conditioning
outlets, mists, splashes and exposure to direct sunlight. It must not be placed in the presence of
flammable gases. Position the MINC such that quick and easy disconnection of the power supply plug is not
impeded.
It is recommended to allow a reasonable amount of space around each MINC to allow for natural air circulation. A
clearance of at least 10 cm is recommended around each device.
The ambient temperature range should be b20°C and +28°C to maintain a device set point between the
ranges of 35°C to 40°C. At a device set point of 37°C, the ambient temperature range can be extended from +18°C
to +32°C.
3.7 Gas supply
3.7.1 Gas mixture required
To maintain the correct operating pH of 7.2 to 7.4 in bicarbonate buffered media the concentration of CO
2
in the
atmosphere in contact with the media must be strictly controlled.
The concentration of CO
2
(expressed as a percentage) required to maintain the correct operating pH is dependent
on the chemical composition and concentration of the media, the altitude at which the MINC is being operated and
the humidification status of the atmosphere in contact with the media.
The correct percentage CO
2
for the desired pH can be determined from the graph provided.
At sea level, the Cook Culture System range of media will give an approximate pH of 7.4 if 5.0% CO
2
is used and 7.3
if 6.0% CO
2
is used in the gas mixture.
Cook Medical recommends the use of 6.0% CO
2
as it promotes a more rapid recovery to an acceptable pH.
Cook Medical recommends a reduced oxygen concentration from the normal atmospheric concentration,
to a more physiological value of 5-8% in the atmosphere in contact with the media, as this may reduce
reactive oxygen species formation.
If using the MINC at an altitude other than sea level, the following graph may be used to find the approximate CO
2
percentage to achieve a pH of 7.3.
% C
O
2
in dr
y gas
% CO
2
required to maintain pH 7.3 vs altitude
2000
3000
4000
5000
6000
1000
0
15
14
13
12
11
10
9
8
7
6
5
Metres above sea level
If a different medium is used or a different pH is required, then the end user will need to determine the appropriate
gas mix for connection to the MINC.
WARNING:
The MINC should
not be used adjacent to or stacked with
other equipment. If adjacent or stacked
use is necessary, the device should be
monitored to verify normal operation
in the configuration in which it will
be used.
WARNING: EXPLOSION
HAZARD.
Do not use the device in
the presence of flammable gases!
WARNING: ELECTRIC SHOCK
HAZARD.
Do not immerse the device!
WARNING:
Ensure that the
appropriate high purity gas mixture
is selected to suit the altitude above
sea level of use and the culture media
being used!
IMPORTANT NOTE:
When using
the Cook Culture System at sea level a
6% CO
2
, 5% O
2
, and 89% N
2
blend high
purity gas mixture is recommended.
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