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K-MINC-1000 Instructions for Use
English
5. Operation of the device
WARNING:
To guarantee safe
operation, it is necessary to carry out
proper care and maintenance of the
device and disposables.
Regular checks to confirm correct
functioning of the device are
recommended!
New and repaired products must be
prepared and tested according to the
manual instructions before use.
IMPORTANT NOTE:
The MINC
measures gas flow internally prior to
the gas entering the humidification
bay. There is no mechanism to verify
correct gas flow into each chamber.
The user must visually ensure the
correct installation of the
humidification flask and gas lines to
ensure unobstructed delivery of gas to
each chamber.
WARNING: BIOLOGICAL
HAZARD.
Do not use a contaminated
humidification flask in the MINC.
It is recommended that the sterile
humidification flask be replaced each
time the sterile water needs refilling,
or replaced regularly with a maximum
period of use being 4 weeks, in order
to avoid bacterial contamination of
contents.
1
This section provides general information about the use of the MINC and Logger Software. Only the clinician/
embryologist can evaluate the clinical factors involved with each patient and determine if the use of this device is
indicated. The clinician/embryologist must decide on the specific technique and procedure that will accomplish
the desired outcome.
5.1 Before use
Prior to commencing the culturing procedure, carry out the following steps:
• Use the temperature selection touch-pads to select the desired chamber temperature (see § 3.11.3).
• Install the humidification flask filled with sterile water as detailed in § 6.1.
• Confirm humidification flask and gas lines are correctly installed.
• Activate the required chambers as per § 3.11.1.
• Use the gas flow selection touch-pads to select the desired gas flow (see § 3.12.4).
• Activate the gas flow (see § 3.12.1). Ensure that gas bubbles are observed in the flask during both the purge cycle
and normal flow.
• Wait a minimum of 4 hours before using to allow for equilibration.
5.2 Insertion of the culture dishes
The MINC has been designed to be used with NUNC® four-well dishes, or NUNC® and FALCON® 35 mm and 60 mm
single round culture dishes.
Four-well dishes or single round dishes may be placed on the chamber base. Ensure that they sit securely in the
grooves designed to accept them. There should be direct contact between the base of the dish and the surface of
the chamber base.
5.3 Patient identification plates
Use the magnetic Patient Identification Plates on the chamber lids to record culture dish contents with a marking
pen. Pen markings can be removed using an alcohol solution.
5.4 Possible condensate formation
During the normal usage of the humidification flask, some condensation may naturally form in the tubing.
Normal
Excessive
Blockage
There are several factors that can cause the formation of condensation:
• Repeated opening and closing of the humidifier chamber lid.
• Leaving the humidifier chamber lid open.
• Fluctuating ambient conditions.
• Draughts.
It is not recommended to obstruct any tubing in any manner.
