2 Preparing for Operation
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September 26, 2013, 715004208 Rev. A
9. In the Time box, specify the number of minutes, from 0.1 through 60.0.
Recommendation:
The default setting is 2.0 minutes. Nevertheless,
prime the isocratic solvent manager until a steady flow exits the vent
tubing (typically 3 minutes).
10. Click Start.
11. Slowly withdraw the syringe plunger.
12. When solvent flows out the vent tubing without bubbles, remove the
syringe from the vent tubing and reconnect the vent tubing to the drip
tray.
Requirements:
•
Ensure that the solvent reservoir contains enough solvent for future
methods.
•
All solvent tubing must contain solvent.
Monitoring LEDs
Light emitting diodes on the ISM indicate its state of functioning.
Power LED
The power LED, on the left-hand side of the ISM’s front panel, indicates when
the ISM is powered-on or powered-off.
Status LED
The flow LED, on the right-hand side of the power LED on the ISM’s front
panel, indicates the flow status.
Power LED indications:
LED mode and color
Description
Green
Power is applied to the ISM.
Black (unlit)
Power is not applied to the ISM.