Priming the ISM
September 26, 2013, 715004208 Rev. A
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Priming the ISM
You must prime the ISM when it or the system that it is part of has never
been used. You must also prime it when you restart a system, after the system
has been idle for more then four hours, or when you change reservoirs or
solvents. During priming, the vent valve moves to the vent position, ensuring
minimal backpressure and directing the flow to waste. The flow rate during
priming is 4 mL/min.
Ensure the solvent reservoirs contain sufficient solvent for adequate priming
and that the waste container can hold all the used solvent. For example, at
4 mL/min, priming for 5 minutes uses about 20 mL of solvent.
Requirement:
You must ensure that the solvent tubing is primed with solvent
in order for the degasser to function properly.
Priming a dry ISM via the console
To prime the ISM:
1. Open the instrument’s front door.
2. Locate the appropriate solvent vent tubing.
3. In the Instrument console, select Isocratic Solvent Manager from the
system tree.
4. In the ISM information window, click Control > Prime solvents.
5. In the Prime Solvents dialog box, select ISM solvent.
Caution:
To prevent salts from precipitating in the system, introduce an
intermediate solvent, such as water, when changing from buffers to
high-organic-content solvents. Be sure to consult the solvent miscibility
tables in the Solvent Considerations section.
Warning:
To avoid spills, empty the waste container at regular
intervals.