Priming the seal-wash system
September 26, 2013, 715004208 Rev. A
65
Note:
Buffered additives must be the highest grade available.
Before priming the plunger seals, ensure the volume of seal-wash is adequate
for priming.
See also:
Controlling Contamination in Ultra Performance LC/MS and
HPLC/MS Systems
on the ACQUITY Chromatography System CD.
Required materials
•
Gloves: clean, powder-free, chemical-resistant
•
Seal wash solution
•
30-mL syringe (startup kit)
•
Tubing adapter (startup kit)
Caution:
• To avoid damaging the solenoid valve seats and seals in the solvent
path, do not use a nonvolatile buffer as the seal-wash solvent.
• To avoid clogging system tubing, ensure the seal-wash solvent is
100% compatible with the mobile phase conditions.
• To avoid spreading contamination, do not recycle seal wash.
• Ensure that seal-wash contains at least 10% organic solvent, a
concentration that prevents microbial growth and ensures that the
seal wash can solubilize the mobile phase.
Mobile phase and seal-wash compatibility:
Mobile phase
Buffer
Seal Wash
Aqueous
Yes/No
Water with at least 10%
organic and is fully miscible
with the mobile phase.
Non-aqueous
Yes/No
Mobile phase without buffer
or 100% IPA if miscible with
mobile phase.
Aqueous/non-aqueous
mixture
Yes
Mobile phase without buffer.
Aqueous/non-aqueous
mixture
No
Mobile phase.