Major components
September 26, 2013, 715004208 Rev. A
19
General guidelines
When using the ACQUITY UPLC and Alliance Flow-splitter kits, observe
these guidelines:
•
If the signal intensity in the QDa detector is low, switch to a restrictor
module with a lower numeric label that allows more analytical flow to
the QDa detector.
•
If the signal intensity in the QDa detector is high, switch to a restrictor
module with a higher numeric label that allows less analytical flow to
the QDa detector.
The following tables provide expected ranges of mobile phase concentration at
the QDa detector (percent mobile phase in make-up flow/mobile phase
mixture entering QDa detector) over a gradient elution for a selected
restrictor module, make-up flow organic, and make-up flow composition. The
tables are valid for a make-up flow rate of 0.3 mL/min and an LC flow rate of
0.6 mL/min over a 0 to 90% organic gradient with methanol, acetonitrile, or
isopropanol.
Expected ranges of mobile phase concentration at the QDa detector
for the ACQUITY UPLC Flow-splitter kit
Restrictor module 10:
Make-up flow
Mobile phase concentration at
QDa detector
Organic
Composition
Minimum %
Maximum %
Isopropanol
10% organic
13.4
25.9
50% organic
3.8
16.0
Methanol
10% organic
14.4
27.0
50% organic
13.8
23.9
Acetonitrile
10% organic
14.6
28.5
50% organic
14.8
30.2