Teledyne ACCQPrep HP125, Installation And Operation Manual

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ACCQPrep HP125
Section 5 PeakTrak
5-26
4. 4.Click the OK button to save your settings and close the
window.
The following menu commands will require a password before
displaying the requested window:
• T
OOLS
> U
SER
M
ANAGEMENT
• H
ELP
> S
ERVICE
• T
OOLS
> S
ET
A
DMINISTRATOR
P
ASSWORD
. This protects
the current password.
5.3 Examples of PeakTrak
actions
In this section you will find instructions for typical PeakTrak
tasks, such as:
• EDITING A METHOD
• ALTERNATIVE WAYS TO CREATE METHOD FILES
• EDITING A DEFAULT METHOD
• DEFINING A GRADIENT
• REAL-TIME GRADIENT EDITING
• MONITORING THE PURITY MEASURE
• VIEWING RUNS
• MANUAL CONTROL OF THE ACCQ Prep
5.3.1 Editing a method Editing a method allows you to tailor the operation of the
ACCQ
Prep system to best separate or purify the compounds of
interest.
PeakTrak has two types of method settings — basic and
advanced.
Basic Method Settings – T h e b a s i c s e t t i n g s a l l o w y o u t o
control the %B gradient mix, flow rate, solvents, and run length.
These settings are part of the M
AIN
window. These settings are
the most frequently changed settings when developing or
improving purification methods.
The %B gradient mix and run length are shown on the plot area.
Refer to Section 5.3.3
Defining a Gradient . for more details. The
other settings are:
• Flow Rate — this defaults to the value created when
setting up the HPLC column but can be changed for
each separation if desired.
• Solvent A and B — Select the desired solvents from the
lists. The solvents are those defined by the C
ONFIGU
-
RATION
window.
Advanced Method Settings – The M
ETHOD
E
DITOR
window
gives you access to advanced settings such as equilibration
volume, initial waste, air purge, peak detection, and peak col-
lection mode.
Equilibration Volume – Equilibration Volume Column equili-
bration is optional, but you may find that separations are more
effective if the column is equilibrated before injecting the sample.