ACCQPrep HP125
Section 5 PeakTrak
5-29
enabled, click the D
ETAILS
button to configure additional
settings. These settings include the slope-based and peak
width options described in
λ
1 and
λ
2 above. Additionally,
you can type or select the minimum and maximum wave-
lengths limits in nanometers. Peak Width for All Wave-
length Detection also determines the length of time before
an automatic auto-zero of baseline occurs. This period is
twice the peak width setting.
External Detector –
Enable this option to use a 0 to 1 volt
analog input signal from an external detector. When
enabled, click the D
ETAILS
button to configure additional
settings. These settings include the slope-based and peak
width options described in
λ
1 and
λ
2 above. The system will
use its internal algorithms to cut detect and cut peaks
based on the analog input signal. Refer to the External
Detector instruction sheet for cable and plumbing require-
ments.
ELS Detector –
Enable this option to use the evaporative
light scattering (ELS) detector on an ACCQ
Prep
Lumen
system. When enabled, click the D
ETAILS
button to config-
ure additional settings:
• Signal Gain: You may modify the gain to scale the
detector signal.
• Slope Based and Threshold: Refer to these features
described in
λ
1 and
λ
2 above.
• Spray Chamber Temperature: The default settings are
30 °C (normal phase default methods) and 15 °C
(reverse phase). This setting may be adjusted from 10 to
60 °C so that detection can be optimized for the solvent
system in use. This setting is limited to a minimum of 5
°C below the ambient temperature.
• Drift Tube Temperature: The default settings are 60 °C
(normal phase default methods) and 60 °C (reverse
phase). This setting may be adjusted from 30 to 90 °C,
but is limited to a range of 5 °C below and 60 °C above
the ambient temperature.
Mass Spectrometer –
(PurIon systems only) Enable this
option to monitor or detect compounds with a PurIon mass
spectrometer system.
• Threshold: A signal level used to detect a peak. This is
based on the baseline noise. The baseline noise is
measured during the first column volume; this is multi-
plied by the value entered in the THRESHOLD control
to generate a trigger value. A peak is collected if the
signal is greater than this trigger value.
• Monitor: Clicking this option prevents fractionation
based on the mass spectrometer signal. Terminate on
Target – Stops the run after all mass spectrometer
detection ions have been detected.
Summary of Contents for ACCQPrep HP125
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Page 6: ...ACCQPrep HP125 Installation and Operation Guide Safety Warnings 6 ...
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Page 128: ...ACCQPrep HP125 Section 6 Maintenance 6 28 ...
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