ACCQPrep HP125
Section 6 Maintenance
6-7
3. Set the Flow Rate to 40 mL/min.
4. Select T
HROUGH
HPLC C
OLUMN
FOR
THE
F
LOW
P
ATH
option.
5. Select N
EXT
for the Pump into Tube # option.
6. Place the B1 Solvent inlet line into a reservoir of methanol,
acetone, or a strong solvent that readily dissolves residual
sample material.
7. Click the P
UMP
S
OLVENT
B button to pump 100% Solvent B
through the bypass tube and into the collection tubes.
8. After three minutes, click the S
TOP
button. Allow the sys-
tem to stand for at least six hours. Overnight is recom-
mended.
9. Return the B1 solvent line to the original solvent container.
10. Perform the Q
UICK
C
LEANING
AS
R
EQUIRED
(Section 6.4.2
and monitor the Raw Lamp Energy. (Figure 6-3)
If the lamp energy is in the green range return the system
to operation. If the lamp energy is red, contact Teledyne
ISCO’s Technical Service department for assistance.
6.5 ACCQ
Prep
HP125
Maintenance
6.5.1 Injection Valve Rotor
Replacement
The following instructions are for the removal and replacement
of the injection valve rotor. (PN: 209009917).
ACCQPrep systems with a serial number starting with 218H or
higher may have spare rotors included with the system. For
these units, access the pump compartment by gripping the edges
of the front panel and pulling forward on both sides (Figure 6-8).
Inside the front cover an envelope contains spare rotors, a hex
key needed for removal and a copy of the replacement instruc-
tions.
Note
The injection valve rotor is a common wear part and is there-
fore not covered under warranty. Since it is a sealing surface it
can be damaged by particulates in the sample. To prolong the
life of the rotor, ensure your sample is properly filtered (<20
µm) to prevent premature failure. Failure is evident by leakage
from an unexpected port or location such as the sample load
port after injection.
Removing the Injection
Valve Rotor
1. Use the supplied
9
/
64
” hex key to remove the 2 screws that
retain the stator (the portion of the inject valve with the
tubing connections). These should be loosened in quarter
turn increments until the tension is removed and a gap
starts to appear (Figure 6-5).
Summary of Contents for ACCQPrep HP125
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Page 6: ...ACCQPrep HP125 Installation and Operation Guide Safety Warnings 6 ...
Page 10: ...ACCQPrep HP125 Table of Contents 4 ...
Page 16: ...ACCQPrep HP125 Section 1 Introduction 1 6 ...
Page 100: ...ACCQPrep HP125 Section 5 PeakTrak 5 36 ...
Page 128: ...ACCQPrep HP125 Section 6 Maintenance 6 28 ...
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