Teledyne ACCQPrep HP125, Installation And Operation Manual

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ACCQPrep HP125
Section 3 Configuration
3-4
3.4.1 Define a Column To define a column:
1. Press the N
EW
button. This will open a keyboard where
you can enter the name of the column.
2. Once the name is complete, press E
NTER
.
3. To complete the column definition, enter the media size
and column dimensions.
4. Select the inside column diameter. A flow rate for a default
method will be selected based on scaling up the linear
velocity of a 4.6 ml analytical column running at 1 ml/min.
5. Select the length of the column. This will provide the sug-
gested default method with ~10 column volumes for the
gradient portion of the separation, along with an initial iso-
cratic portion, a strong solvent portion to wash the column
and a portion that returns the column to a suggested stor-
age fluid concentration.
6. The pressure limit is set by default to 3000 psi (207 bar)
which is a typical limit for preparative HPLC columns, but
can be changed for each column to a suitable pressure not
to exceed the system pressure limit of 6000 psi.
7. Press S
AVE
, to save the default method associated with the
column.
To customize the just created method, press D
EFINE
M
ETHODS
followed by E
DIT
to view and modify all the method parameters.
Further information can be found in Section 5.2.2.
3.4.2 Delete a Column To delete a column:
1. Ensure the column to be deleted is not selected in the col-
umn position field(s).
2. Press the column drop down and select the column you
would like to delete.
3. Press the D
ELETE
button.
4. In some cases you may get an error message stating the
method is currently in use. To address this, leave the C
ON
-
FIGURATION
screen and go the to R
UN
screen and select a
different column on the R
UN
screen.