Teledyne ACCQPrep HP125, Installation And Operation Manual

Page: 54 / 142

ACCQPrep HP125
Section 4 Operation
4-8
Sample Name – Allows you to name each sample. Naming is
optional. If left blank, the sample is named based on the date and
time the separation was started.
Column (drop down menu) – I f a C o l u m n S e l e c t o r Va l v e
Module (CSV-4) is installed, this menu allows you to select a sep-
aration column and associated method for use. If a CSV-4 is not
installed, this menu allows you to select different methods that
may be associated with the configured and installed separation
column.
Method – Once a column has been selected, or if there is only
one column defined on the system, the default method created for
that column is automatically loaded. To change this method,
touch the method name and a list of methods associated with the
column will appear. These methods will be listed in the order
they were created. Alternatively, select C
USTOMIZE
C
URRENT
M
ETHOD
or B
ROWSE
to populate this field.
Select C
USTOMIZE
C
URRENT
M
ETHOD
to create a modified version
of the method for use in the current queue. To use this modified
method, exit the method editor and select S
AVE
when prompted.
A new method named “Temporary 1” will be created. This
method will be used for all injections of the current sample and
will be discarded after the sample is complete. All method
parameters are saved with each separation. If you would like to
save the modified method for later use, select S
AVE
A
S
before you
exit the method editor and create a unique name for the new
method.
Sample Position – This field indicates where the sample for
this separation is located. If the sample size is too large for a
single vial, create a second line in the queue and access the
second vial.
Sample Volume – Enter the amount of sample to be separated.
Typically this amount is slightly greater than the amount of
sample to ensure all of the sample gets purified. This information
is used in conjunction with the N
UMBER OF
I
NJECTIONS
column to
calculate the volume of each injection.
Number of Injections – Used in conjunction with the S
AMPLE
V
OLUMES
column to determine the size of each injection.
Start Tube – Allows you to optimize rack usage by sharing
racks for multiple samples or optimizing work flow by placing
each sample’s fraction into individual racks to support multiple
users.
3. Once the queue is complete, you can select the R
UN
tab and
press the P
LAY
button or select the “=” menu on the first
unfinished run and select the S
TART
R
UN
option. In either
case, the R
UN
R
EQUIREMENTS
screen will be shown. This
allows you to accept or modify the starting rack or tube for
fraction collection.
4. While a separation is in process, the Q
UEUE
screen can be
accessed to add samples to the queue. Samples that have
been completed cannot be edited. They can be viewed, how-