Teledyne ACCQPrep HP125, Installation And Operation Manual

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ACCQPrep HP125
Section 5 PeakTrak
5-28
Peak Detection – This section of the window contains option
buttons to enable and disable various peak detection options.
When an option is enabled, the window also allows you to modify
the setting details for that peak detection option. All enabled
options will be displayed on the chromatogram. T
Note
You can select up to four peak detection options (excluding the
External option) on the ACCQ
Prep systems. If using more than
one option, such as
λ
1 with
λ
2, the system considers a peak
to be present when either option is true (a logical OR).
λ
1
and
λ
2
(wavelength 1 and 2) – Enable these options to
use and configure primary and secondary wavelength detec-
tion. When enabled, type or select the peak detection wave-
length in nanometers, then click the D
ETAILS
button to
configure additional settings described below.
Slope Based – this option to enable Slope-based peak detec-
tion. When enabled, peaks will be recorded if the slope algo-
rithm indicates a peak within the Peak Width setting.
Peak Width – Select the average peak width setting. Peak
widths are measured at the baseline. The slope detector will
detect peak widths ranging rom about 0.2 to 2 times the
peak width setting. For example, if you entered a peak
width of 1 minute, the range would be 12 seconds to 2 min-
utes. For best operation, the peak width should be set to
just over the average peak width being separated. For
instance, if the average peak width is 45 seconds, you
should enter a peak width of 1 minute. For most flash chro-
matography, 1 minute is a good starting point for a peak
width.
Threshold – Select this option to enable Threshold peak
detection. When enabled, peaks will be recorded if the
Absorbance Units (AU) value is exceeded. Type or select the
Absorbance Units value to be used for Threshold detection.
Note
If both Slope and Threshold peak detection methods are
checked, the system considers a peak to be present when any
one condition is met. This logical OR operator means that the
system will cut a peak when either the Slope condition is true,
or when the Threshold condition is true.
Monitor – Enable this option to only use the detection
source as a monitor. When enabled, the detection source
will be displayed as a trace on the gradient plot area, but
will not be used to cut peaks.
All Wavelength Detection – Enable this option to detect
peaks within a user-selected range of wavelengths. When