Teledyne ACCQPrep HP125, Installation And Operation Manual

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ACCQPrep HP125
Section 5 PeakTrak
5-34
• UV-visible spectrum (UV-Vis systems only). Clicking in
the Chromatogram window displays a mass spectrum at
that point during the run.
• E
XTRACT
M
ETHOD
button — Click the E
XTRACT
button
to load a new method file based on the parameters for
the run you are viewing.
• C
LOSE
button — Click the C
LOSE
button when you are
done viewing the run.
General instructions for reading the data – Most ele-
ments on the window are color-keyed to help you locate the
tubes containing the peaks of interest. Below each collected
peak there is a color bar that matches a tube in the map on
the left. The tube map provides a visual representation of
the tubes that contain the peaks of interest. If you wish to
identify the tube by number, refer to the table below the
rack diagram.
If the run used multiple racks, the P
REVIOUS
and N
EXT
R
ACK
buttons are active. Click these buttons to scroll
through the available racks. The currently displayed rack is
identified by letter which is shown below the rack .
Reading the Chromatogram – The plot area displays the
following:
· The red absorbance trace produced by the system’s
peak detector. Absorbance units (also shown in red)
that correspond to this trace are shown on the left
Y-axis.
· Purple absorbance and green purity measurement
traces may be visible if you are monitoring a second
wavelength.
· Green traces are from ELSD (Lumen systems) or
external detectors.
· The PurIon traces are color coded with the selected
masses below the graph y-axis.
· The blue gradient curve that was used during the
run. The% Solvent B scale is shown in blue on the
right Y-axis.
· The X-axis depicts the run time, shown as minutes.
· Vertical lines appear at intervals along the X-axis.
These lines indicate collection tube changes. To
prevent the plot area from being obscured by tube
change marks, PeakTrak may limit the number of
marks.