Agilent Technologies 1100 Series, Reference Manual, Page: 46 / 346

48 1100 Series FD Reference Manual
2
First Steps with the Fluorescence Detector
Step 3: Set up routine methods
In routine analysis, sample matrices can have a significant influence on
retention times. For reliable results, sample preparation must be thorough to
avoid interferences or LC methods must be rugged enough. With difficult
matrices, simultaneous multi-wavelength detection offers more reliability than
timetable-controlled wavelength switching. The Agilent 1100 Series FLD can,
in addition, acquire fluorescence spectra while it records the detector signals
for quantitative analysis. Therefore qualitative data are available for peak
confirmation and purity checks in routine analysis.
Table 9 Conditions for Figure 15 and Figure 16 on page 47
Column Zorbax SB, 2 x 50 mm, PNA, 5 µm
Mobile phase A = water; B = acetonitrile
Gradient 0 minutes, 5%
10 minutes, 15%
Flow rate 0.4 ml/min
Column temperature 35
°
C
Injection volume 5 µl
FLD settings PMT 12,
response time 4 s,
step size 5 nm
Ex 265 nm and 430 nm
Em 540 nm