14
03/06
Scanning a Z stack
•
Select
Z Stack
in the
Scan Control
window.
•
Select
Frame
if necessary.
The
Z Settings
panel appears.
•
Select
Mark First/Last
on the
Z Settings
panel.
•
Click on the
XY cont
button.
A continuous XY-scan of the set focus position will
be performed.
•
Use the focusing drive of the microscope to
focus on the upper position of the specimen
area where the Z Stack is to start.
•
Click on the
Mark First
button to set the upper
position of the Z Stack.
•
Then focus on the lower specimen area where
the recording of the Z Stack is to end.
•
Click on the
Mark Last
button to set this lower
position.
•
Click on
X:Y:Z=1:1:1
button to set the Z-
interval in such a way that the voxel has
identical dimensions in the X-, Y- and Z-
directions (cube).
•
Click on the
Start
button to start the recording
of the Z Stack.
Fig. 22
Scan Control window, Z Stack settings
Содержание LSM 510
Страница 1: ...LSM 510 LSM 510 DuoScan LSM 510 META LSM 510 META DuoScan Release 4 0 March 2006...
Страница 62: ...IMPORTANT NOTES FOR CHAPTER 4 LSM 510 DuoScan Carl Zeiss LSM 510 META Duo Scan 4 II B 45 0021 e 03 06...
Страница 442: ...Programming for LSM LSM 510 DuoScan Carl Zeiss Events LSM 510 META DuoScan 5 18 B 45 0021 e 03 06...
Страница 526: ...3D FOR LSM LSM 510 DuoScan Carl Zeiss Functions LSM 510 META DuoScan 7 60 B 45 0021 e 03 06...
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Страница 566: ...MULTIPHOTON LASER SCANNING MICROSCOPY Carl Zeiss Contents LSM 510 META NLO 9 4 B 45 0021 e 03 06...
Страница 591: ...LSM 510 and LSM 510 META Laser Scanning Microscopes Brief Operating Manual Release 4 0 March 2006...
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