7. Quality Control
116
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
7.4.5.2 AlphaScreen Uniformity:
Pipette the reagents into the wells of a Greiner 384-well plate (white, flat bottom)
according to the plate layout below.
Material:
AlphaScreen Omnibeads #6760626D (PerkinElmer)
Greiner 384-well plate, flat bottom, white
Phosphate-buffered saline (PBS)
100 µl p tips
Omnibeads dilution series:
Dilute the Omnibeads stock solution 1:2000 in PBS by adding 3 µl of the stock
solution (5 mg/ml) to 5997 µl PBS (yielding a solution of 2.5 µg/ml).
Plate layout:
Pipette 100 µl of the Omnibeads dilution the highlighted wells of the microplate
(as shown in the plate layout).
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1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
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22
23
24
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Filling volume: 100 µl/well Omnibeads dilution (2.5 µg/ml)
Measurement Parameters:
Before pipetting the plate, prepare instrument for measurement:
Parameters:
Measurement mode:
AlphaScreen/AlphaLISA
Excitation time:
100 ms
Integration time:
300 ms
Temperature correction: activated
Plate definition file:
GRE384fw.pdfx, whole plate
Start measurement immediately after pipetting!
