7. Quality Control
120
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
7.4.6.3 Absorbance Linearity 384-well Plate:
Pipette 100 µl of the reagents into the wells of a Greiner 384-well plate
(flat bottom, transparent) as described under Plate Layout.
Material:
Greiner 384-well plate, flat bottom, transparent
Orange G (120 µg/l; 60 µg/l, 40 µg/l, 20 µg/l, 10 µg/l) diluted in water
Distilled water (Blank)
100 µl p tips
Plate layout:
100 µl/well
Measurement Parameters OG:
Measurement mode:
Absorbance
Measurement wavelength: 492 nm
Number of flashes:
50
Plate definition file:
GRE384ft.pdfx
Part of the plate:
A1 – H6
Evaluation:
Calculate the average of each concentration (and blank) and subtract blank from
samples. Plot the blank subtracted mean measurement values of Orange G
against the concentration values. Calculate the coefficient of correlation (r
2
).
1
2
3
4
5
6
7
8
9
10
…
…
A
120 mg/l Oran
ge G
60 mg/l Orang
e G
40 mg/l Orang
e G
20 mg/l Orang
e G
10 mg/l Orang
e G
Blank
B
C
D
E
F
G
H
…
