5. Operating the INFINITE M1000 PRO
60
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
For each selected label, one or two wells of the defined plate range can be used
for the z-position optimization. Select the well(s) and click ‘
Scan
’ to start the z-
optimization: The z-positioning option ‘
Max S/B Ratio
’ requires the measurement
of two wells, one filled with the fluorophore of interest (signal) and one filled with
buffer (blank). Both wells are scanned and the resulting signal and blank curves
are shown in the graph. The z-position may now be set to the maximum signal-
to-blank (S/B) ratio.
Figure 31
Note
When the option ‘Max S/B Ratio’ is used, the sample well is first
measured with optimal gain and the very same gain value is then
applied to the second measurement with the blank well. Therefore, both
signal and blank curves are directly comparable.
The z-position for each selected label can be defined manually. The vertical
yellow bar of the graph can be moved to the desired z-position. Upon clicking
‘
Apply
’, the selected z-position will be automatically applied to i-control script
and used for the subsequent measurement.
