4. Optical System
36
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
Filter Wheel PMT
The filter wheel contains wavelength specific optical filters, which are necessary
to block undesired diffraction orders produced by the optical gratings. The filters
are set automatically.
PMT Detector
A photo-multiplier tube (PMT) is used for the detection of the low light levels
associated with fluorescence. The dedicated fluorescence PMT of the
INFINITE
M1000 PRO
is sensitive up to the near infrared (NIR) while still having low dark
current. Electronic circuitry uses analog to digital conversion of PMT output
current. Adjusting the PMT gain enables measurement of a wide range of
concentrations in lower or higher concentration domains. For details, see chapter
5.3.3 Instrument Parameters.
4.1.4 Luminescence
Scan
The
INFINITE M1000 PRO
is capable of recording emission spectra of
luminescent signals by using the fluorescence top or bottom emission optics.
The light emitted by the luminescent sample is collected by the top/bottom lens
system, coupled into the emission fiber bundle and guided to the emission
monochromator. The emission monochromator is used to select any wavelength
from 280 nm to 850 nm of the luminescent signal. After light of the selected
wavelength passes the emission monochromator, it is focused onto the detector
(PMT: photo-multiplier tube). The results are given in relative luminescence units
(RLU). The bandwidth can be selected from 5 nm to 20 nm in 1 nm increments.
The integration time can be selected from 1 ms to 1 s.
