7.
Quality
Control
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
109
7.4.3.2 FP Precision Fluorescein 1536-well Plate:
Pipette 10 µl of the reagents into the wells of a Greiner 1536-well plate (black, flat
bottom) according to the Plate Layout.
Material:
Greiner 384-well plate, flat bottom, black
10 µl pipette (8-channel) + tips
1 nM Fluorescein
0.01 nM NaOH (=Blank)
High Polarization Standard (Invitrogen)
Plate Layout:
<>
1
…
5
…
9
…
13
…
17
…
21
…
25
…
29
…
33
…
37
…
41
…
45
…
A
F P F B F B F B F B F B
...
E
F P F B F B F B F B F B
...
I
F B F B F B F B F B F B
…
M
F B F B F B F B F B F B
…
Q
F B F B F B F B F B F B
…
U
F B F B F B F B F B F B
…
Y
F B F B F B F B F B F B
…
AC
F B F B F B F B F B F B
…
(F….10 µl 1 nM Fluorescein; B…..10 µl 0.01 M NaOH = Blank, P…10 µl
Invitrogen HighPolStandard)
Measurement Parameters:
Measurement mode:
Fluorescence Polarization
Excitation wavelength:
470 nm
Emission wavelength:
525 nm
Emission Bandwidth:
20 nm
Gain:
‘Optimal’
Lag
time: 0
µs
Number of reads:
10 (= 100 ms integration time)
Settle
time:
50
ms
Plate definition file:
GRE1536fb.pdfx
Z-Position:
Calculated from Well A1
G-Factor Reference:
Calibrated from well A1; ref. value = 20 mP;
Reference blank:
I5; sample blank is same as reference blank
Evaluation:
Calculate the lower detection limit [mP] = 'precision':
FP Precision (mP) = Stdev
F
Stdev
F
Standard deviation of wells filled with Fluorescein (except reference well)
