5. Operating the INFINITE M1000 PRO
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Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
Weekly/Periodical Maintenance:
The fluid path must be cleaned weekly to remove precipitates such as salts,
eliminate bacterial growth, etc.:
The following maintenance protocol is designed for two available injectors (A+B),
but can be performed with only one injector (A) as well.
Follow these steps to clean the pump/injector system with 70% EtOH (ethanol):
1. Depending on the user’s application flush the system thoroughly with buffer
or distilled water before washing with 70% EtOH.
2. Place an empty microplate in the reader.
3. Prime both injectors with 70% EtOH.
4. Set up a software script consisting of a Plate stripe (with a plate definition file
corresponding to the inserted type of microplate), Part of the Plate (Well A1)
and a Dispense stripe.
5. Select injectors A and B and set the dispense volume to 5 µl for each
injector.
6. Set the Refill Mode to ‘Standard’ and the Refill Volume to the maximum
possible value (e.g. 995 µl for a 1000 µl syringe).
7. Start the script and leave the syringes filled for at least 30 minutes.
8. After the 30-minute period, backflush all the fluid from the syringes and
tubes into a small waste container.
9. Wash the injector system with distilled or deionized water.
10. Clean the end of the injector needles carefully with a cotton swab soaked in
70 % ethanol or isopropanol.
11. For prolonged storage, repeat steps 1-7 with distilled or deionized water and
leave the fluid pathways filled for storage.
